PDF DatasheetHuman MMP 8 Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant MMP 8 based upon sequence from Human
Host: QP10789 protein expressed in E. Coli.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of MMP 8 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes Used as a standard for analyzing mammalian collagenase activity.
Bioactivity Data: 100 units/ml after activation with APMA by solution assay method. One unit of collagenolytic activity is defined as the cleavage of 1µg of collagen per minute by the solution method.
Amino Acid Sequence: MFSLKTLPFL LLLHVQISKA FPVSSKEKNT KTVQDYLEKF YQLPSNQYQS TRKNGTNVIV EKLKEMQRFF GLNVTGKPNE ETLDMMKKPR CGVPDSGGFM LTPGNPKWER TNLTYRIRNY TPQLSEAEVE RAIKDAFELW SVASPLIFTR ISQGEADINI AFYQRDHGDN SPFDGPNGIL AHAFQPGQGI GGDAHFDAEE TWTNTSANYN LFLVAAHEFG HSLGLAHSSD PGALMYPNYA FRETSNYSLP QDDIDGIQAI YGLSSNPIQP TGPSTPKPCD PSLTFDAITT LRGEILFFKD RYFWRRHPQL QRVEMNFISL FWPSLPTGIQ AAYEDFDRDL IFLFKGNQYW ALSGYDILQG YPKDISNYGF PSSVQAIDAA VFYRSKTYFF VNDQFWRYDN QRQFMEPGYP KSISGAFPGI ESKVDAVFQQ EHFFHVFSGP RYYAFDLIAQ RVTRVARGNK WLNCRYG
Purity: Greater than 90% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: The MMP-8 protein solution (100 units/ml) in 0.05M Tris-HCl buffer, pH 7.6, 0.2M NaCl, 5mM CaCl2, 0.0025% NaN3 and 0.1% BSA.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered clear solution.
| Recombinant Human MMP 8 General Information | |
|---|---|
 |
|
| Alternate Names | |
| Neutrophil collagenase, EC 3.4. 24.34, Matrix metalloproteinase-8, MMP-8, PMNL collagenase, PMNL-CL, HNC, CLG1. | |
| Molecular Weight | |
| 75 kDa | |
| Chromosomal Location | |
| q22.2 on chromosome 11 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | MMP8 |
| Entrez Gene ID | 4317 |
| UniProt ID(s) | P22894 |
| COSMIC ID Link(s) | MMP8 |
| KEGG Gene ID(s) | hsa:4317 |
| General Description of Recombinant Human MMP 8. | |
| Human Matrix Metalloproteinase-8 produced in E. Coli is a single, non-glycosylated, polypeptide chain having a molecular mass of 75 kDa. The MMP-8 is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
There are no reviews yet.