PDF DatasheetHuman NNT1 Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant NNT1 based upon sequence from Human
Host: QP10794 protein expressed in E. Coli.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of NNT1 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized NNT1 in sterile 18M-cm H2O not less than 0.5 mg/ml, which can then be further diluted to other aqueous solutions.
Bioactivity Data: The ED50, as measured in a cell proliferation assay using human TF?1 cells transfected with human CNTF R?, is less than 15ng/ml.
Amino Acid Sequence: MLNRTGDPGP GPSIQKTYDL TRYLEHQLRS LAGTYLNYLG PPFNEPDFNP PRLGAETLPR ATVDLEVWRS LNDKLRLTQN YEAYSHLLCY LRGLNRQAAT AELRRSLAHF CTSLQGLLGS IAGVMAALGY PLPQPLPGTE PTWTPGPAHS DFLQKMDDFW LLKELQTWLW RSAKDFNRLK KKMQPPAAAV TLHLGAHGF
Purity: Greater than 95.0% as determined by SDS-PAGE.
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: NNT-1 protein was lyophilized from a 0.2µm filtered solution in Acetonitrile and TFA.
Storage Conditions: Lyophilized Neurotrophin-1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution NNT1 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
| Recombinant Human NNT1 General Information | |
|---|---|
| Alternate Names | |
| Cardiotrophin-Like Cytokine Factor 1, Novel Neurotrophin-1 , BSF3, CLC, CRLF1 Associated Cytokine-Like Factor 1, B-Cell Stimulating Factor 3, B-Cell-Stimulating Factor 3, BSF-3, NNT-1, NNT1, Neurotrophin-1/B-Cell Stimulating Factor-3, Cold-Induced Sweating Syndrome 2, B-Cell Stimulatory Factor 3, CISS2, NR6, CLCF1. | |
| Molecular Weight | |
| 22 kDa | |
| Chromosomal Location | |
| q13.2 on chromosome 11 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | CLCF1 |
| Entrez Gene ID | 23529 |
| UniProt ID(s) | Q9UBD9 |
| COSMIC ID Link(s) | CLCF1 |
| KEGG Gene ID(s) | hsa:23529 |
| General Description of Recombinant Human NNT1. | |
| Human Neurotrophin-1 (28-225) produced in E. Coli is a single, non-glycosylated, polypeptide chain containing 199 amino acids and having a molecular mass of 22kDa. The NNT-1 is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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