PDF DatasheetOvine Placental Lactogen Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant Placental Lactogen based upon sequence from Ovine
Host: QP10827 protein expressed in E. Coli.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of Placental Lactogen was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized Placental Lactogen in sterile 18MΩ.cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Bioactivity Data: Placental Lactogen Ovine is biologically active as evidenced by inducing proliferation of Nb2 cells.
Amino Acid Sequence: MAPASSHREH QWTCNLVRGS RLLLLLVVSN LILCQGQAQH PPYCRNQPGK CQIPLQSLFD RATTVANYNS KLAGEMVNRF DEQYGQGINS ESKVINCHTS SITTPNSKAE AINTEDKILF KLVISLLHSW DEPLHHAVTE LANSKGTSPA LLTKAQEIKE KAKVLVDGVE VIQKRIHPGE KNEPYPVWSE QSSLTSQDEN VRRVAFYRLF HCLHRDSSKI YTYLRILKCR LTSCET
Purity: Greater than 98.0% as determined by:(a) Analysis by SEC-HPLC. (b) Analysis by SDS-PAGE.
Concentration of Ovine Placental Lactogen Protein: UV spectroscopy at 280 nm using the absorbency value of 0.85 as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: The protein was lyophilized from a concentrated (1 mg/ml) solution with 0.02-0.03% NaHCO3.
Storage Conditions: Lyophilized Placental Lactogen Ovine Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Placental Lactogen should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
| Recombinant Ovine Placental Lactogen General Information | |
|---|---|
| Alternate Names | |
| Chorionic Somatomammotropin Hormone 1, CSH1, CS-1, hCS, PL. | |
| Molecular Weight | |
| 23 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | P16038 |
| General Description of Recombinant Ovine Placental Lactogen. | |
| Placental Lactogen Ovine Recombinant, is a single polypeptide chain containing 199 amino acids and an additional Ala at the N-terminus having a molecular mass of 23 kDa. Placental Lactogen Recombinant is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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