PDF DatasheetHuman Procalcitonin Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant Procalcitonin based upon sequence from Human
Host: QP10847 protein expressed in E. Coli.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of Procalcitonin was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized procalcitonin sterile 18MΩ.cm H2O at 100 µg/ml, which can then be further diluted to other aqueous solutions.
Bioactivity Data: Human Procalcitonin was tested using Biomerieux reagents on the Min-Vidas. The assay was run at 80ng/ml with a typical recovery of 80 percent.
Amino Acid Sequence: APFRSALESS PADPATLSED EARLLLAALV QDYVQMKASE LEQEQEREGS SLDSPRSKRC GNLSTCMLGT YTQDFNKFHT FPQTAIGVGA PGKKRDMSSD LERDHRPHVS MPQNAN
Purity: Greater than 95.0% as determined by SDS-PAGE.
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: Lyophilized from 10mM sodium phosphate pH 7.5.
Storage Conditions: Lyophilized procalcitonin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution procalcitonin should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Selected References
A sandwich assay for procalcitonin detection in POCT applications.Publication:Advanced Biomedical and Clinical Diagnostic Systems VII, edited by Anita Mahadevan-Jansen,Tuan Vo-Dinh, Warren S. Grundfest, Proc. of SPIE Vol. 7169, 716905© 2009 SPIE · CCC code: 1605-7422/09/$18 · doi: 10.1117/12.810265Link:http://144.206.159.178/ft/CONF/16425379/16425383.pdf
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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