PDF Datasheetother PROK Tritirachium album Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant PROK Tritirachium album based upon sequence from other
Host: QP10849 protein expressed in Yeast.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of PROK Tritirachium album was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized Proteinase-K in 20mM Tris-HCl (pH 7.4~8.0), 1mM CaCl2, 50% glycerol not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Bioactivity Data: 34 Units/mg protein. One unit is defined as the amount of enzyme that will hydrolyze urea-denatured hemoglobin to produce color equivalent to 1.0 mol tyrosine per min at 37°C, pH 7.5 (color by Folin-Ciocalteu reagent).
Amino Acid Sequence: MRLSVLLSLL PLALGAPAVE QRSEAAPLIE ARGEMVANKY IVKFKEGSAL SALDAAMEKI SGKPDHVYKN VFSGFAATLD ENMVRVLRAH PDVEYIEQDA VVTINAAQTN APWGLARISS TSPGTSTYYY DESAGQGSCV YVIDTGIEAS HPEFEGRAQM VKTYYYSSRD GNGHGTHCAG TVGSRTYGVA KKTQLFGVKV LDDNGSGQYS TIIAGMDFVA SDKNNRNCPK GVVASLSLGG GYSSSVNSAA ARLQSSGVMV AVAAGNNNAD ARNYSPASEP SVCTVGASDR YDRRSSFSNY GSVLDIFGPG TSILSTWIGG STRSISGTSM ATPHVAGLAA YLMTLGKTTA ASACRYIADT ANKGDLSNIP FGTVNLLAYN NYQA
Purity: Greater than 99% as determined by SDS-PAGE.
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: The Proteinase-K was lyophilized without any additives.
Storage Conditions: Recombinant Proteinase-K although stable at room temp for 1 week, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered lyophilized powder.
| Recombinant other PROK Tritirachium album General Information | |
|---|---|
| Alternate Names | |
| Proteinase K (EC:3.4. 21.64), Endopeptidase K, Tritirachium alkaline proteinase, PROK. | |
| Molecular Weight | |
| 40.3 kDa | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | PROK |
| UniProt ID(s) | P06873 |
| COSMIC ID Link(s) | PROK |
| General Description of Recombinant other PROK Tritirachium album. | |
| Recombinant Tritirachium album Proteinase-K expressed in yeast containing 285 amino acids having a MW of 29.3 kDa is purified by standard chromatography techniques. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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