PDF DatasheetOvine Prolactin Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant Prolactin based upon sequence from Ovine
Host: QP10851 protein expressed in E. Coli.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of Prolactin was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized Prolactin in sterile 18MΩ.cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Bioactivity Data: Is fully biologically active as evidenced by inducing proliferation of Nb2 cells.
Amino Acid Sequence: MDSKGSAQKG SRLLLLLVVS NLLLCQGVVS TPVCPNGPGN CQVSLRDLFD RAVMVSHYIH NLSSEMFNEF DKRYAQGKGF ITMALNSCHT SSLPTPEDKE QAQQTHHEVL MSLILGLLRS WNDPLYHLVT EVRGMKGVPD AILSRAIEIE EENKRLLEGM EMIFGQVIPG AKETEPYPVW SGLPSLQTKD EDARHSAFYN LLHCLRRDSS KIDTYLKLLN CRIIYNNNC
Purity: Greater than 98.0% as determined by:(a) Analysis by SEC-HPLC. (b) Analysis by SDS-PAGE.
Concentration of Ovine Prolactin Protein: Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 0.85 as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of Prolactin as a Reference Standard.
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: The protein was lyophilized from a concentrated (1 mg/ml) solution with 0.0045mM NaHCO3.
Storage Conditions: Lyophilized Prolactin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Prl should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
| Recombinant Ovine Prolactin General Information | |
|---|---|
| Alternate Names | |
| Mammotropin, Luteotropic hormone, Luteotropin, PRL. | |
| Molecular Weight | |
| 23 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | P01240 |
| General Description of Recombinant Ovine Prolactin. | |
| Prolactin Ovine Recombinant produced in E. Coli is a single, non-glycosylated polypeptide chain containing 199 amino acids and having a molecular mass of 23 kDa. The Prolactin n is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Selected References
1.Prolactin Treatment Improves Engraftment and Function of Transplanted Pancreatic Islets.Publication:Published online before print December 18, 2008, doi: 10.1210/en.2008-1318 Endocrinology April 1, 2009 vol. 150 no. 4 1646-1653 .Link:http://endo.endojournals.org/content/150/4/1646.full2.ISLET ENDOTHELIAL CELLS AND PANCREATIC ?-CELL PROLIFERATION: STUDIES IN VITRO AND DURING PREGNANCY IN ADULT RATS.Publication:Endocrinology. First published January 26, 2006 as doi:10.1210/en.2005-0997.Link:http://endo.endojournals.org/content/early/2006/01/26/en.2005-0997.full.pdf3.Prolactin Treatment Improves Engraftment and Function of Transplanted Pancreatic Islets.Publication:Endocrinology. First published ahead of print December 18, 2008 as doi:10.1210/en.2008-1318.Link:http://endo.endojournals.org/content/early/2008/12/18/en.2008-1318.full.pdf4. Islet Endothelial Cells and Pancreatic -CellProliferation: Studies in Vitro and during Pregnancy in Adult Rats.Publication:Published online before print January 26, 2006, doi:10.1210/en.2005-0997 Endocrinology May 1, 2006vol. 147no. 52315-2324.Link:http://endo.endojournals.org/content/147/5/2315.full.pdf+html
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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