PDF DatasheetHuman PTH Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant PTH based upon sequence from Human
Host: QP10855 protein expressed in E. Coli.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of PTH was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized PTH in sterile 18MΩ.cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Bioactivity Data: The activity calculated by UMR106 cell/cAMP method corresponding to a specific activity of 10,000 Units/mg.
Amino Acid Sequence: LMHNLGKHLN SMERVEWLRK KLQDVHNF
Purity: Greater than 97.0% as determined by:(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: The protein (1 mg/ml) was lyophilized after extensive dialyses against 20mM PBS pH 7.0 and 150mM NaCl.
Storage Conditions: Lyophilized Parathyrin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PTH should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
| Recombinant Human PTH General Information | |
|---|---|
| Alternate Names | |
| PTH/PTHrP receptor antagonist, PTHrP analog, PTHR, MGC138426, MGC138452. | |
| Molecular Weight | |
| 3.4 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | Q03431 |
| General Description of Recombinant Human PTH. | |
| Human Parathyroid Hormone produced in E. Coli is a single, non-glycosylated, polypeptide chain containing 28 amino acids and having a molecular mass of 3380 Dalton. The PTH is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
There are no reviews yet.