PDF DatasheetHuman RLN3 Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant RLN3 based upon sequence from Human
Host: QP10859 protein expressed in E. Coli.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of RLN3 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized RLN3 in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Bioactivity Data: The ED50, as measured by cAMP accumulation in human THP-1 cells, is less than 17.5ng/ml.
Monomer or Dimer: Dimer
Amino Acid Sequence: A chain: DVLAGLSSSC CKWGCSKSEI SSLCB chain: RAAPYGVRLCG REFIRAVIFT CGGSRW
Purity: Greater than 95.0% as determined by SDS-PAGE.
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: Lyophilized from a 0.2µm filtered solution in Acetonitrile and TFA.
Storage Conditions: Lyophilized RLN3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Relaxin-3 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
| Recombinant Human RLN3 General Information | |
|---|---|
| Alternate Names | |
| Relaxin 3, Prorelaxin H3, RXN3, Insl7, ZINS4, H3, Relaxin 3 (H3), Relaxin-3, RLN3. | |
| Molecular Weight | |
| 2.5 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | Q8WXF3 |
| General Description of Recombinant Human RLN3. | |
| Human Relaxin-3 produced in E. Coli is a disulfide-linked heterodimeric, non-glycosylated, polypeptide chain containing 24 amino acids for A chain and 27 amino acids for B chain and having a molecular mass of 2.5kDa for A chain and 3kDa for B chain. The Relaxin-3 is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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