PDF DatasheetMouse sRANKL Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant sRANKL based upon sequence from Mouse
Host: QP10869 protein expressed in E. Coli.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of sRANKL was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized sRANKL in sterile 18MΩ.cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Bioactivity Data: The ED50, as measured by its ability to induce osteoclast differentiation of RAW 264.7 mouse monocyte/macrophage cells, is less than 2ng/ml.
Amino Acid Sequence: Lys158-Asp316, with an N-terminal Met, Accession # Q3TWY5
Purity: Greater than 95.0% as determined by SDS-PAGE.
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: sRANKL protein was lyophilized from a 0.2µm filtered solution in NaH2PO4, NaCl and EDTA.
Storage Conditions: Lyophilized sRANKL although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution sRANKL should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
| Recombinant Mouse sRANKL General Information | |
|---|---|
| Alternate Names | |
| Soluble Receptor Activator of NFkB Ligand, TNFSF11, TRANCE, TNF-related activation-induced cytokine, OPGL, ODF, Osteoclast differentiation factor, Tumor necrosis factor ligand superfamily member 11, Receptor activator of nuclear factor kappa B ligand, RANKL, Osteoprotegerin ligand, CD254 antigen, sRANKL, sOdf. | |
| Molecular Weight | |
| 19 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | Q3TWY5 |
| General Description of Recombinant Mouse sRANKL. | |
| Mouse sRANKL produced in E. coli is a single, non-glycosylated polypeptide chain (158-316 AA) having a molecular mass of 19kDa. The sRANKL is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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