PDF Datasheetother Staphylokinase Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant Staphylokinase based upon sequence from other
Host: QP10870 protein expressed in E. Coli.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of Staphylokinase was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized SAKin sterile 18MΩ.cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Bioactivity Data: The biological activity measured by the ability of fibrin lysis in agarose plate was found to be 50,000IU/mg.
Amino Acid Sequence: MLKRSLLFLT VLLLLFSFSS ITNEVSASSS FDKGKYKKGD DASYFEPTGP YLMVNVTGVD GKGNELLSPH YVEFPIKPGT TLTKEKIEYY VEWALDATAY KEFRVVELDP SAKIEVTYYD KNKKKEETKS FPITEKGFVV PDLSEHIKNP GFNLITKVVI EKK
Purity: Greater than 95.0% as determined by:(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: Lyophilized from a 0.2µm filtered concentrated solution in PBS, pH 7.4.
Storage Conditions: Lyophilized SAK although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution SAK should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
| Recombinant other Staphylokinase General Information | |
|---|---|
| Alternate Names | |
| Staphylokinase, SakSTAR, Neutral proteinase, Protease III, SAK. | |
| Molecular Weight | |
| 16 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | P68802 |
| General Description of Recombinant other Staphylokinase. | |
| Staphylokinase Recombinant produced in E. Coli is a non-glycosylated polypeptide chain containing 136 amino acids and having a molecular weight of 16kDa. The Staphylokinase is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Selected References
Staphylokinase production by clinical Staphylococcus aureus strainsPublications: Pol J Microbiol 56 (2007): 97-102.Link: http://www.researchgate.net/publication/6187424_Staphylokinase_production_by_clinical_Staphylococcus_aureus_strains
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
There are no reviews yet.