PDF DatasheetPorcine Thrombin Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant Thrombin based upon sequence from Porcine
Host: QP10878 protein expressed in Native Protein.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of Thrombin was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized Thrombin in sterile 18MΩ.cm H2O.
Bioactivity Data: One unit is defined as the amount of enzyme needed to cleave 1 mg of fusion protein in 16 hours to 95% completion at 20°C in a buffer containing 25mM Tris-HCl, pH 8.4, 150mM NaCl, and 2.5mM CaCl2.
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: Lyophilized Powder from 20mM PBS, pH 7.4 containing 0.9% NaCl.
Storage Conditions: Lyophilized Thrombin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IPF1 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Limitations and Performance Guarantee
enQuire Bio’s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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