PDF DatasheetDISC: Rabbit TNF a Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant TNF a based upon sequence from Rabbit
Host: QP10882 protein expressed in E. Coli.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of TNF a was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized Tumor Necrosis Factor-alpha in sterile 18MΩ.cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Bioactivity Data: The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D is less than 0.03ng/ml, corresponding to a Specific Activity of 30,000,000 IU/mg.
Amino Acid Sequence: MSTESMIRDV ELAEGPLPKK AGGPQGSKRC LCLSLFSFLL VAGATTLFCL LHFRVIGPQE EEQSPNNLHL VNPVAQMVTL RSASRALSDK PLAHVVANPQ VEGQLQWLSQ RANALLANGM KLTDNQLVVP ADGLYLIYSQ VLFSGQGCRS YVLLTHTVSR FAVSYPNKVN LLSAIKSPCH RETPEEAEPM AWYEPIYLGG VFQLEKGDRL STEVNQPEYL DLAESGQVYF GIIAL
Purity: Greater than 95% as determined by:(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: TNF-alpha Rabbit was lyophilized after extensive dialysis against 20mM PBS, pH 7.4, 300mM NaCl.
Storage Conditions: Lyophilized Tumor Necrosis Factor-a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TNF-a should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
| Recombinant Rabbit TNF a General Information | |
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| Alternate Names | |
| Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, TNF, TNFA, TNFSF2. | |
| Molecular Weight | |
| 17.4 kDa | |
| Chromosomal Location | |
| On chromosome 12 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | TNF |
| Entrez Gene ID | 100009088 |
| UniProt ID(s) | P04924 |
| COSMIC ID Link(s) | TNF |
| KEGG Gene ID(s) | ocu:100009088 |
| General Description of Recombinant Rabbit TNF a. | |
| Tumor Necrosis Factor-a Rabbit Recombinant consists of three identical polypeptide chains of 158 amino acids combined to form a compact, bell-shaped homotrimer. TNF-alpha was produced in E. Coli is a non-glycosylated, polypeptide chain having a molecular mass of 17.4 kDa for the individual subunit. The TNF-alpha is purified by standard chromatographic techniques. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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