PDF DatasheetMouse TNFR2 Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant TNFR2 based upon sequence from Mouse
Host: QP10886 protein expressed in E. Coli.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of TNFR2 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized TNFR2 in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Bioactivity Data: The ED50 as determined by its ability to inhibit the TNF-? mediated cytotoxicity in the L-929 cells is less than 0.2µg/ml, corresponding to a specific activity of > 5000IU/mg in the presence of 0.25ng/mL of rHuTNF-a.
Amino Acid Sequence: MPAQVAFTPY APEPGSTCRL REYYDQTAQM CCSKCSPGQH AKVFCTKTSD TVCDSCEDST YTQLWNWVPE CLSCGSRCSS DQVETQACTR EQNRICTCRP GWYCALSKQE GCRLCAPLRK CRPGFGVARP GTETSDVVCK PCAPGTFSNT TSSTDICRPH QICNVVAIPG NASMDAVCTS TSPT
Purity: Greater than 97.0% as determined by SDS-PAGE.
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: TNFR2 protein was lyophilized from a 0.2µm filtered concentrated solution in PBS, pH 7.4.
Storage Conditions: Lyophilized TNFR2 although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution TNFR2 should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
| Recombinant Mouse TNFR2 General Information | |
|---|---|
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| Alternate Names | |
| Tumor necrosis factor receptor superfamily member 1B, Tumor necrosis factor receptor 2, TNF-R2, Tumor necrosis factor receptor type II, TNF-RII, TNFR-II, p75, p80 TNF-alpha receptor, CD120b, Tnfrsf1b, Tnfr-2, Tnfr2, TNFBR, TNFR80, TNFRII, TNF-R75, TNF-R-II, TNF-alphaR2, TNFalpha-R2. | |
| Molecular Weight | |
| 20 kDa | |
| Chromosomal Location | |
| E1 on chromosome 4 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | Tnfrsf1b |
| Entrez Gene ID | 21938 |
| UniProt ID(s) | P25119 |
| COSMIC ID Link(s) | Tnfrsf1b |
| KEGG Gene ID(s) | mmu:21938 |
| General Description of Recombinant Mouse TNFR2. | |
| Mouse TNFR2 produced in E. coli is a single, non-glycosylated polypeptide chain containing 184 amino acids and having a molecular mass of 20kDa. The TNFR2 is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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