PDF DatasheetMouse TNFSF14 Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant TNFSF14 based upon sequence from Mouse
Host: QP10890 protein expressed in E. Coli.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of TNFSF14 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized TNFSF14 in sterile 100mM HAc not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Bioactivity Data: The ED50 as determined by a cytotoxicity assay using human HT-29 cells is less than 2µg/ml, corresponding to a specific activity of > 500 IU/mg in the presence of murine anti-polyHistidine monoclonal antibody and rHuIFN-g.
Amino Acid Sequence: DGGKGSWEKL IQDQRSHQAN PAAHLTGANA SLIGIGGPLL WETRLGLAFL RGLTYHDGAL VTMEPGYYYV YSKVQLSGVG CPQGLANGLP ITHGLYKRTS RYPKELELLV SRRSPCGRAN SSRVWWDSSF LGGVVHLEAG EEVVVRVPGN RLVRPRDGTR SYFGAFMV
Purity: Greater than 96.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: TNFSF14 protein was lyophilized from a 0.2µm filtered concentrated solution in PBS pH 7.4.
Storage Conditions: Lyophilized TNFSF14 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TNFSF14 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
| Recombinant Mouse TNFSF14 General Information | |
|---|---|
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| Alternate Names | |
| Tumor necrosis factor ligand superfamily member 14, CD258, Tnfsf14, Light. | |
| Molecular Weight | |
| 18.4 kDa | |
| Chromosomal Location | |
| D on chromosome 17 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | Tnfsf14 |
| Entrez Gene ID | 50930 |
| UniProt ID(s) | Q9QYH9 |
| COSMIC ID Link(s) | Tnfsf14 |
| KEGG Gene ID(s) | mmu:50930 |
| General Description of Recombinant Mouse TNFSF14. | |
| Mouse TNFSF14 produced in E. Coli is a single, non-glycosylated, polypeptide chain containing 168 amino acids and having a molecular mass of 18.4kDa. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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