PDF Datasheetother TrxR Yeast Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant TrxR Yeast based upon sequence from other
Host: QP10895 protein expressed in E. Coli.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of TrxR Yeast was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes
Definition of Unit (for specific activity): One unit equals the change in absorbance at 412 nm per minute at 25°C using 0.2mM NADPH containing 5mM DTNB (pH 7.0).
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized NTR in sterile 18MΩ.cm H2O.
Bioactivity Data: The specific activity was found to be 5.8 IU/mg.
Amino Acid Sequence: MKNLTNEIYD VIIVGSGPAG LTSAIYTARA GLKTVIYEQS APGGKLIKTD LIENYPGFET IQGPDLASKM YLQALSLNAS VEFVGVDSIF KNADDIFEIS LTNGEIKKAY SVILATGTQE NKLGIKGEDE LYGKGVSYCA VCDGAFYKNK PVAVVGGGYS AVQEAMYLSQ LVDKVYLIVR RDVFRADANK VAKLKAQPNV EFLLKSQVKE IHGAEKVESL TITTPDGEKK LVVSAIFPYI GSTPLIDSVK HLCIENEKGY IPTNDKMQTE IKGLFVAGDV RDVPLRQIAI ACGDGAIAGQ MAVEYVQEVK
Purity: Greater than 95.0% as determined by SDS-PAGE.
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: Each mg of protein contains 20mM phosphate buffer pH 7.4.
Storage Conditions: NTR although stable at 4°C for 3 weeks, should be stored desiccated below -18°C. Please prevent freeze thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
| Recombinant other TrxR Yeast General Information | |
|---|---|
| Alternate Names | |
| Thioredoxin Reductase (NADPH), NTR, TrxR. | |
| Molecular Weight | |
| 36 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | Q6MS43 |
| General Description of Recombinant other TrxR Yeast. | |
| Thioredoxin Reductase (NADPH) Yeast Recombinant produced in E. Coli is a single, non-glycosylated, polypeptide chain having a molecular mass of 36 kDa. Thioredoxin Reductase is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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