PDF DatasheetHuman Trypsin-2 Bioactive Protein Product Attributes
Product Type: Bioactive Protein
Recombinant Trypsin-2 based upon sequence from Human
Host: QP10899 protein expressed in E. Coli.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of Trypsin-2 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: Bioactive
Application Notes Trypsin digestion: the suggested ratio is 1:50 to 1:1000 (w/w).
Definition of Unit (for specific activity): One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a reaction volume of 3.0ml at pH7.6 and 25°C, with BAEE as a substrate (1cm light path).
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized Human Trypsin in sterile 1mM HCl or 50mM HAC not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Bioactivity Data: 2,800 units/mg.
Specific Activity: 2,800 units/mg.
Amino Acid Sequence: MNLLLILTFV AAAVAAPFDD DDKIVGGYIC EENSVPYQVS LNSGYHFCGG SLISEQWVVS AGHCYKSRIQ VRLGEHNIEV LEGNEQFINA AKIIRHPKYN SRTLDNDILL IKLSSPAVIN SRVSAISLPT APPAAGTESL ISGWGNTLSS GADYPDELQC LDAPVLSQAE CEASYPGKIT NNMFCVGFLE GGKDSCQGDS GGPVVSNGEL QGIVSWGYGC AQKNRPGVYT KVYNYVDWIK DTIAANS
Purity: Greater than 90% as determined by SDS-PAGE.
Endotoxin Levels: < 1.0 EU per ug protein as determined by the LAL method.
Buffer: The protein was lyophilized without any additives.
Storage Conditions: Recombinant Human Trypsin although stable at room temp for 1 week, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered lyophilized powder.
| Recombinant Human Trypsin-2 General Information | |
|---|---|
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | P07478 |
| General Description of Recombinant Human Trypsin-2. | |
| Recombinant Human Trypsin-2 expressed in E. Coli having an MW of 24kDa is purified by standard chromatography techniques. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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