PDF Datasheetother 1F8 Chagas Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant 1F8 Chagas based upon sequence from other
Host: QP10919 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of 1F8 Chagas was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized 1F8 Chagas protein in sterile distilled pyrogen free water at 100µg/ml, which can then be further diluted to other aqueous solutions.
Bioactivity Data: Untested
Amino Acid Sequence: MGACGSKGST SDKGLASDKD GKKAKDRKEA WERIRQAIPR EKTAEAKQRR IELFKKFDKN ETGKLCYDEV HSGCLEVLKL DEFTPRVRDI TKRAFDKARA LGSKLENKGS EDFVEFLEFR LMLCYIYDFF ELTVMFDEID ASGNMLVDEE ELKRAVPKLE AWGAKVEDPA ALFKELDKNG TGSVTFDEFA AWASAVKLDA DGDPDNVPES A
Purity: Protein is >90% pure as determined by SDS PAGE.
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: 1F8 Chagas antigen was lyophilized from 20mM Hepes, 300mM NaCl, pH 8.0, and varying 284mM imidazole.
Storage Conditions: Store lyophilized 1F8 Chagas antigen at 2-8°C. After reconstitution, store at -20°C. Prevent freeze/thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
| Recombinant other 1F8 Chagas General Information | |
|---|---|
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | P07749 |
| General Description of Recombinant other 1F8 Chagas. | |
| Recombinant Chagas 1F8 protein (Epitope of the flagellar calcium-binding protein FCABP of Trypanosoma cruzi) produced in E. Coli, is a polypeptide chain having a total of 234 a. a (includes his tag). The protein is fused to a 6-His tag having a total MW of 25.8kDa and a theoretical pI of 5.39. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
There are no reviews yet.