PDF DatasheetHuman ANGPTL3 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant ANGPTL3 based upon sequence from Human
Host: QP11018 protein expressed in HEK 293, E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of ANGPTL3 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes
Recommended Reconstitution Instructions: It is recommended to add deionized water to a working concentration of 0.5 mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Bioactivity Data: Untested
Amino Acid Sequence: SRIDQDNSSF DSLSPEPKSR FAMLDDVKIL ANGLLQLGHG LKDFVHKTKG QINDIFQKLN IFDQSFYDLS LQTSEIKEEE KELRRTTYKL QVKNEEVKNM SLELNSKLES LLEEKILLQQ KVKYLEEQLT NLIQNQPETP EHPEVTSLKT FVEKQDNSIK DLLQTVEDQY KQLNQQHSQI KEIENQLRRT SIQEPTEISL SSKPRAPRTT PFLQLNEIRN VKHDGIPAEC TTIYNRGEHT SGMYAIRPSN SQVFHVYCDV ISGSPWTLIQ HRIDGSQNFN ETWENYKYGF GRLDGEFWLG LEKIYSIVKQ SNYVLRIELE DWKDNKHYIE YSFYLGNHET NYTLHLVAIT GNVPNAIPEN KDLVFSTWDH KAKGHFNCPE GYSGGWWWHD ECGENNLNGK YNKPRAKSKP ERRRGLSWKS QNGRLYSIKS TKMLIHPTDS ESFEHHHHHH
Purity: Greater than 95.0% as determined by SDS-PAGE.
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: Filtered (0.4µm) and lyophilized from 0.5 mg/ml in 0.05M phosphate buffer and 0.075M NaCl, pH 7.4.
Storage Conditions: Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time.
Physical Appearance: Filtered White lyophilized (freeze-dried) powder.
| Recombinant Human ANGPTL3 General Information | |
|---|---|
| Alternate Names | |
| Angiopoietin 5, ANGPT5, ANGPTL3, Angiopoietin Like Protein 3. | |
| Molecular Weight | |
| 52.6 kDa | |
| Chromosomal Location | |
| p31.3 on chromosome 1 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | ANGPTL3 |
| Entrez Gene ID | 27329 |
| UniProt ID(s) | Q9Y5C1 |
| COSMIC ID Link(s) | ANGPTL3 |
| KEGG Gene ID(s) | hsa:27329 |
| General Description of Recombinant Human ANGPTL3. | |
| Human ANGPTL3 produced in HEK cells is a single, glycosylated, polypeptide chain (a. a 17-460) containing a total of 450 amino acids, having a molecular mass of 52.6kDa (calculated) and fused to a 6 aa His tag at C-Terminus. The Human ANGPTL3 is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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