PDF DatasheetHuman APOA5 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant APOA5 based upon sequence from Human
Host: QP11046 protein expressed in HEK 293, E. Coli.
Available Tags: Flag Tag, His
Protein Construction: A cDNA sequence encoding the sequence of APOA5 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes
Recommended Reconstitution Instructions: It is recommended to add deionized water to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it on cell culture.
Bioactivity Data: Untested
Amino Acid Sequence: RKGFWDYFSQ TSGDKGRVEQ IHQQKMAREP ATLKDSLEQD LNNMNKFLEK LRPLSGSEAP RLPQDPVGMR RQLQEELEEV KARLQPYMAE AHELVGWNLE GLRQQLKPYT MDLMEQVALR VQELQEQLRV VGEDTKAQLL GGVDEAWALL QGLQSRVVHH TGRFKELFHP YAESLVSGIG RHVQELHRSV APHAPASPAR LSRCVQVLSR KLTLKAKALH ARIQQNLDQL REELSRAFAG TGTEEGAGPD PQMLSEEVRQ RLQAFRQDTY LQIAAFTRAI DQETEEVQQQ LAPPPPGHSA FAPEFQQTDS GKVLSKLQAR LDDLWEDITH SLHDQGHSHL GDPAAADYKD DDDK
Purity: Greater than 70% as determined by SDS-PAGE.
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: APOA5 Human was filtered (0.4µm) and lyophilized from 0.5 mg/ml supplied in 20mM TRIS and 50mM NaCl, pH 7.5
Storage Conditions: Store lyophilized APOA5 Human at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted APOA5 Human can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
Physical Appearance: Sterile White Lyophilate (freeze-dried powder) from 0.2 µm filtered solution
| Recombinant Human APOA5 General Information | |
|---|---|
| Alternate Names | |
| Apolipoprotein A-V, Apo-AV, ApoA-V, Apolipoprotein A5, Regeneration-associated protein 3, APOA5, RAP3, APOAV. | |
| Molecular Weight | |
| 41.2 kDa | |
| Chromosomal Location | |
| q23.3 on chromosome 11 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | APOA5 |
| Entrez Gene ID | 116519 |
| UniProt ID(s) | Q6Q788 |
| COSMIC ID Link(s) | APOA5 |
| KEGG Gene ID(s) | hsa:116519 |
| General Description of Recombinant Human APOA5. | |
| Human APOA5 Flag-Tagged Fusion Protein is 40.1 kDa protein containing 354 amino acid residues of the APOA5 Human and 11 additional amino acid residues of flag Tag. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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