PDF DatasheetHuman APOD Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant APOD based upon sequence from Human
Host: QP11050 protein expressed in HEK 293.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of APOD was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes
Recommended Reconstitution Instructions: It is recommended to add 200µl deionized water to a working concentration of 0.5 mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Bioactivity Data: Untested
Amino Acid Sequence: QAFHLGKCPN PPVQENFDVN KYLGRWYEIE KIPTTFENGR CIQANYSLME NGKIKVLNQE LRADGTVNQI EGEATPVNLT EPAKLEVKFS WFMPSAPYWI LATDYENYAL VYSCTCIIQL FHVDFAWILA RNPNLPPETV DSLKNILTSN NIDVKKMTVT DQVNCPKLSH HHHHH
Purity: Greater than 95.0% as determined by SDS-PAGE.
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: Filtered (0.4µm) and lyophilized from 0.5 mg/ml in 0.05M phosphate buffer and 0.075M NaCl, pH 7.4.
Storage Conditions: Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time.
Physical Appearance: Filtered White lyophilized (freeze-dried) powder.
| Recombinant Human APOD General Information | |
|---|---|
 |
|
| Alternate Names | |
| Apolipoprotein D, Apo-D, ApoD. | |
| Molecular Weight | |
| 20.1 kDa | |
| Chromosomal Location | |
| q29 on chromosome 3 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | APOD |
| Entrez Gene ID | 347 |
| UniProt ID(s) | P05090 |
| COSMIC ID Link(s) | APOD |
| KEGG Gene ID(s) | hsa:347 |
| General Description of Recombinant Human APOD. | |
| Human Apolipoprotein-D produced in HEK cells is a single, glycosylated, polypeptide chain (aa 21-189) containing a total of 175 amino acids, having a molecular mass of 20.1kDa (calculated) and fused to a 6 aa His tag at C-Terminus. The Human APOD is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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