PDF DatasheetAprotinin Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant Aprotinin based upon sequence from Bos taurus.
Host: QP11058 protein expressed as native protein.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of Aprotinin was constructed and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes
Definition of Unit (for specific activity): 1 Unit corresponds to 1 biological kallikrein inhibitor unit (KIU)1 TIU =1,300 KIU.
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized Aprotinin in sterile 18MΩ.cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Bioactivity Data:
Specific Activity: 6,438 KIU (Kallikrein Inactivator Units) per mg, 3.58 TIU/mg.
Amino Acid Sequence: MKMSRLCLSV ALLVLLGTLA ASTPGCDTSN QAKAQRPDFC LEPPYTGPCK ARIIRYFYNA KAGLCQTFVY GGCRAKRNNF KSAEDCMRTC GGAIGPWENL
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: The protein (1 mg/ml) was lyophilized with no additives.
Storage Conditions: Lyophilized Aprotinin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Aprotinin should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
| Recombinant other Aprotinin General Information | |
|---|---|
| Alternate Names | |
| Pancreatic trypsin inhibitor, Basic protease inhibitor, BPI, BPTI, Aprotinin, AP. | |
| Molecular Weight | |
| 10.9 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | P00974 |
| COSMIC ID Link(s) | – |
| General Description of Recombinant other Aprotinin. | |
| Aprotinin is a natural proteinase inhibitor polypeptide consisting of fifty-eight amino acids {C284H432N84O79S7} arranged in a single polypeptide chain, cross-linked by three disulfide bridges and having a molecular mass of 6512. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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