PDF DatasheetHuman BD5 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant BD5 based upon sequence from Human
Host: QP11149 protein expressed in E. Coli.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of BD5 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized Beta Defensin-5 in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Bioactivity Data: Untested
Amino Acid Sequence: GLDFSQPFPS GEFAVCESCK LGRGKCRKEC LENEKPDGNC RLNFLCCRQR I
Purity: Greater than 95.0% as determined by:(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: Lyophilized from a 0.2µm filtered concentrated solution in PBS, pH 7.4.
Storage Conditions: Lyophilized Beta Defensin-5 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BD5 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
| Recombinant Human BD5 General Information | |
|---|---|
| Alternate Names | |
| Beta-defensin 105, eta-defensin 5, BD-5, DEFB-5, Defensin, beta 105, DEFB105A, BD5, DEFB105, DEFB5, DEFB105B. | |
| Molecular Weight | |
| 5.8 kDa | |
| Chromosomal Location | |
| p23.1 on chromosome 8 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | DEFB105A; DEFB105B |
| Entrez Gene ID | 504180; 245908 |
| UniProt ID(s) | Q8NG35 |
| COSMIC ID Link(s) | DEFB105A; DEFB105B |
| KEGG Gene ID(s) | hsa:245908; hsa:504180 |
| General Description of Recombinant Human BD5. | |
| Human BD5 produced in E. Coli is a single, non-glycosylated, polypeptide chain containing 51 amino acids and having a molecular mass of 5.8 kDa. The Beta Defensin-5 is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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