PDF DatasheetHuman C1QTNF1 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant C1QTNF1 based upon sequence from Human
Host: QP11230 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of C1QTNF1 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes
Recommended Reconstitution Instructions: Add 0.1M Acetate buffer pH4 to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10?g/ml. In higher concentrations the solubility of this antigen is limited. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Bioactivity Data: Untested
Amino Acid Sequence: MKHHHHHHAS RVPHVQGEQQ EWEGTEELPS PPDHAERAEE QHEKYRPSQD QGLPASRCLR CCDPGTSMYP ATAVPQINIT ILKGEKGDRG DRGLQGKYGK TGSAGARGHT GPKGQKGSMG APGERCKSHY AAFSVGRKKP MHSNHYYQTV IFDTEFVNLY DHFNMFTGKF YCYVPGLYFF SLNVHTWNQK ETYLHIMKNE EEVVILFAQV GDRSIMQSQS LMLELREQDQ VWVRLYKGER ENAIFSEELD TYITFSGYLVKHATEP
Purity: Greater than 95% as determined by SDS PAGE.
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: Human C1QTNF1 was lyophilized from 50mM Acetate Buffer pH 4.
Storage Conditions: Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
Physical Appearance: Sterile White Lyophilate (freeze-dried powder) from 0.2 µm filtered solution
| Recombinant Human C1QTNF1 General Information | |
|---|---|
| Alternate Names | |
| GIP, CTRP1, ZSIG37, FLJ90694, C1QTNF1, Complement C1q Tumor Necrosis Factor-Related Protein 1, G protein-coupled receptor-interacting protein. | |
| Molecular Weight | |
| 30.45 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | Q9BXJ1 |
| General Description of Recombinant Human C1QTNF1. | |
| Human C1QTNF1 produced in E. Coli is a single, non-glycosylated, Polypeptide chain containing 266 amino acids and having a molecular mass of 30.45 kDa. The protein contains an extra His tag at N-terminus. The C1QTNF1 amino acid sequence is identical to UniProtKB/Swiss-Prot entry Q9BXJ1 amino acids 26281. The C1QTNF1 is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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