PDF DatasheetRat CALB1 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant CALB1 based upon sequence from Rat
Host: QP11257 protein expressed in E. Coli.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of CALB1 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes CABP28K can be used for immunoblots, absorption experiments in immunohistochemistry, radioimmunoassay and intracellular injection. For adsorption we suggest the following procedureA- Dilute 1 µl of the monoclonal antibody against calbindin D-28k in 5 ml of the usual buffer for immunohistochemistry (final dilution 1:5’000). B- Add 1 µg of the recombinant protein to 1 ml of the diluted antibody solution and mix well. C- Incubate for at least 6 hours in the cold. D- Apply to tissue-sections and incubate for 3 days. E- Complete the immunohistochemical reaction as usual (biotinylated second antibody, ABC-complex, DAB). As a result, the immunostaining should be strongly reduced or even completely prevented.
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized CABP28K in sterile 18MΩ.cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Bioactivity Data: Untested
Amino Acid Sequence: MAESHLQSSL ITASQFFEIW LHFDADGSGY LEGKELQNLI QELLQARKKA GLELSPEMKT FVDQYGQRDD GKIGIVELAH VLPTEENFLL LFRCQQLKSC EEFMKTWRKY DTDHSGFIET EELKNFLKDL LEKANKTVDD TKLAEYTDLM LKLFDSNNDG KLELTEMARL LPVQENFLLK FQGIKMCGKE FNKAFELYDQ DGNGYIDENE LDALLKDLCE KNKQELDINN ISTYKKNIMA LSDGGKLYRT DLALILSAGD N
Purity: Greater than 90% as determined by SDS-PAGE.
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: The protein was lyophilized from a concentrated solution (1 mg/ml) containing 50mM NaHCO3.
Storage Conditions: Lyophilized CABP28K although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CABP28K should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
| Recombinant Rat CALB1 General Information | |
|---|---|
| Alternate Names | |
| Calbindin, Vitamin D-dependent calcium-binding protein, avian-type, Calbindin D28, D-28K, Spot 35 protein, Calb1, CaBP28K, MGC93326. | |
| Molecular Weight | |
| 30 kDa | |
| Chromosomal Location | |
| q13 on chromosome 5 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | Calb1 |
| Entrez Gene ID | 83839 |
| UniProt ID(s) | P07171 |
| COSMIC ID Link(s) | Calb1 |
| KEGG Gene ID(s) | rno:83839 |
| General Description of Recombinant Rat CALB1. | |
| Recombinant Rat Calbindin-1 produced in E. Coli. The Rat CALB1 is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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