PDF DatasheetMouse CD14 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant CD14 based upon sequence from Mouse
Host: QP11301 protein expressed in CHO, Insect.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of CD14 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized Mouse CD14 in sterile 18MΩ.cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Bioactivity Data: Untested
Amino Acid Sequence: MERVLGLLLL LLVHASPAPP EPCELDEESC SCNFSDPKPD WSSAFNCLGA ADVELYGGGR SLEYLLKRVD TEADLGQFTD IIKSLSLKRL TVRAARIPSR ILFGALRVLG ISGLQELTLE NLEVTGTAPP PLLEATGPDL NILNLRNVSW ATRDAWLAEL QQWLKPGLKV LSIAQAHSLN FSCEQVRVFP ALSTLDLSDN PELGERGLIS ALCPLKFPTL QVLALRNAGM ETPSGVCSAL AAARVQLQGL DLSHNSLRDA AGAPSCDWPS QLNSLNLSFT GLKQVPKGLP AKLSVLDLSY NRLDRNPSPD ELPQVGNLSL KGNPFLDSES HSEKFNSGVV TAGAPSSQAV ALSGTLALLL GDRLFV
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: CD14 was lyophilized from a concentrated protein solution (1 mg/ml) containing phosphate-buffered saline, pH 7.2.
Storage Conditions: Lyophilized Mouse CD14 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Mouse CD14 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
| Recombinant Mouse CD14 General Information | |
|---|---|
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| Alternate Names | |
| Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein, CD14. | |
| Molecular Weight | |
| 39.2 kDa | |
| Chromosomal Location | |
| B2 on chromosome 18 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | Cd14 |
| Entrez Gene ID | 12475 |
| UniProt ID(s) | P10810 |
| COSMIC ID Link(s) | Cd14 |
| KEGG Gene ID(s) | mmu:12475 |
| General Description of Recombinant Mouse CD14. | |
| The CD14 is produced from mouse CD14 transfected CHO-cells in serum free medium. Before transfection the complete mouse CD14-cDNA was amplified by PCR and cloned into expression vector p-POL-DHFR). The recombinant mouse CD-14 was purified by His-tag and controlled by SDS page. The myeloid differentiation antigen CD14 acts as the major receptor for bacterial LPS. The dominant form of the recombinant wildtype CD14 is the 50 kDa protein. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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