PDF DatasheetHuman CENPB Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant CENPB based upon sequence from Human
Host: QP11388 protein expressed in Insect.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of CENPB was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes
Plate Coating Instructions: 0.15-0.375 µg/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination.
Other Applications: 1. Binds IgG-type human auto-antibodies. 2. Standard ELISA test (checker-board analysis of positive/negative sera panels including CDC international reference sera).
Bioactivity Data: Untested
Amino Acid Sequence: MGPKRRQLTF REKSRIIQEV EENPDLRKGE IARRFNIPPS TLSTILKNKR AILASERKYG VASTCRKTNK LSPYDKLEGL LIAWFQQIRA AGLPVKGIIL KEKALRIAEE LGMDDFTASN GWLDRFRRRH GVVSCSGVAR ARARNAAPRT PAAPASPAAV PSEGSGGSTT GWRAREEQPP SVAEGYASQD VFSATETSLW YDFLPDQAAG LCGGDGRPRQ ATQRLSVLLC ANADGSEKLP PLVAGKSAKP RAGQAGLPCD YTANSKGGVT TQALAKYLKA LDTRMAAESR RVLLLAGRLA AQSLDTSGLR HVQLAFFPPG TVHPLERGVV QQVKGHYRQA MLLKAMAALE GQDPSGLQLG LTEALHFVAA AWQAVEPSDI AACFREAGFG GGPNATITTS LKSEGEEEEE EEEEEEEEEG EGEEEEEEGE EEEEEGGEGE ELGEEEEVEE EGDVDSDEEE EEDEESSSEG LEAEDWAQGV VEAGGSFGAY GAQEEAQCPT LHFLEGGEDS DSDSEEEDDE EEDDEDEDDD DDEEDGDEVP VPSFGEAMAY FAMVKRYLTS FPIDDRVQSH ILHLEHDLVH VTRKNHARQA GVRGLGHQS
Purity: Greater than 95% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: CENPB is supplied at a concentration of in 20mM HEPES buffer pH 7.6, 250mM NaCl and 20% glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered liquid formulation.
| Recombinant Human CENPB General Information | |
|---|---|
| Alternate Names | |
| Major centromere autoantigen B, Centromere protein B, CENP-B, CENPB. | |
| Molecular Weight | |
| 80 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | P07199 |
| General Description of Recombinant Human CENPB. | |
| Human CENPB produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 80kDa. CENPB is expressed with a -6x His tag at N-terminus and purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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