PDF DatasheetHuman CRISP1 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant CRISP1 based upon sequence from Human
Host: QP11505 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of CRISP1 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized CRISP1 in sterile 18MΩ.cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Bioactivity Data: Untested
Amino Acid Sequence: MKHHHHHHAS KKKSARDQFN KLVTDLPNVQ EEIVNIHNAL RRRVVPPASN MLKMSWSEEA AQNARIFSKY CDMTESNPLE RRLPNTFCGE NMHMTSYPVS WSSVIGVWYS ESTSFKHGEW TTTDDDITTD HYTQIVWATS YLIGCAIASC RQQGSPRYLY VCHYCHEGND PETKNEPYKT GVPCEACPSN CEDKLCTNPC IYYDEYFDCD IQVHYLGCNH STTILFCKAT CLCDTEIK
Purity: Greater than 85% as determined by SDS-PAGE.
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: The protein was lyophilized from a 0.5 mg/ml solution containing 20mM TRIS, 50mM NaCl, pH 7.5.
Storage Conditions: Lyophilized CRISP1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CRISP1 should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
| Recombinant Human CRISP1 General Information | |
|---|---|
| Alternate Names | |
| Cysteine-rich secretory protein 1, ARP, CRISP-1, AEGL1, Acidic epididymal glycoprotein-like 1, HSCRISP1D, HSCRISP1G, HUMARP, AEG-like protein, cysteine-rich secretory protein-1 delta. | |
| Molecular Weight | |
| 27 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | P54107 |
| General Description of Recombinant Human CRISP1. | |
| The CRISP1 Protein Human produced in E. Coli is a single, non-glycosylated polypeptide chain containing 238 amino acids (Lys 22 – Lys 249) having a molecular mass of 27kDa. CRISP1 is fused to 10 amino acids His-Tag at N-terminus and purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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