PDF DatasheetRat Cyclophilin A Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant Cyclophilin A based upon sequence from Rat
Host: QP11578 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of Cyclophilin A was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes
Recommended Reconstitution Instructions: It is recommended to add deionized water to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. Cyclophilin-A is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Bioactivity Data: Untested
Amino Acid Sequence: MKHHHHHHASVNPTVFFDIT ADGEPLGRVC FELFADKVPK TAENFRALST GEKGFGYKGS SFHRIIPGFM CQGGDFTRHN GTGGKSIYGE KFEDENFILK HTGPGILSMA NAGPNTNGSQ FFICTAKTEW LDGKHVVFGK VKEGMSIVEA MERFGSRNGK TSKKITISDC GQL
Purity: Greater than 95.0% as determined by SDS-PAGE.
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: Cyclophilin-A was filtered (0.4µm) and lyophilized from 0.5 mg/ml solution in phosphate buffered saline and 5%(w/v) trehalose.
Storage Conditions: Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
Physical Appearance: Filtered White lyophilized (freeze-dried) powder.
| Recombinant Rat Cyclophilin A General Information | |
|---|---|
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| Alternate Names | |
| Peptidyl-prolyl cis-trans isomerase A, EC:5.2. 1.8, PPIase A, Cyclophilin A, Cyclosporin A-binding protein, Rotamase A, p1B15, p31, Ppia. | |
| Molecular Weight | |
| 19 kDa | |
| Chromosomal Location | |
| p21 on chromosome 14 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | Ppia |
| Entrez Gene ID | 100360977; 25518 |
| UniProt ID(s) | P10111 |
| COSMIC ID Link(s) | Ppia |
| KEGG Gene ID(s) | rno:100360977; rno:25518 |
| General Description of Recombinant Rat Cyclophilin A. | |
| Rat Cyclophilin-A produced in E. Coli is a single, non-glycosylated, polypeptide chain (Val2-Leu164) containing 173 amino acids including a 10 aa His tag at N-terminus. The total calculated molecular mass is 19kDa. | |
Limitations and Performance Guarantee
enQuire Bio’s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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