PDF DatasheetHuman DAG1 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant DAG1 based upon sequence from Human
Host: QP11595 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of DAG1 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes
Recommended Reconstitution Instructions: It is recommended to add 200µl deionized water to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. DAG1 is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Bioactivity Data: Untested
Amino Acid Sequence: MKHHHHHHASHWPSEPSEAV RDWENQLEAS MHSVLSDLHE AVPTVVGIPD GTAVVGRSFR VTIPTDLIAS SGDIIKVSAA GKEALPSWLH WDSQSHTLEG LPLDTDKGVH YISVSATRLG ANGSHIPQTS SVFSIEVYPE DHSELQSVRT ASPDPGEVVS SACAADEPVT VLTVILDADL TKMTPKQRID LLHRMRSFSE VELHNMKLVP VVNNRLFDMS AFMAGPGNAK KVVENGALLS WKLGCSLNQN SVPDIHGVEA PAREGAMSAQ LGYPVVGWHI ANKKPPLPKR VRR
Purity: Greater than 90.0% as determined by SDS-PAGE.
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: DAG1 filtered (0.4 µm) and lyophilized from 0.5 mg/ml in 0.05M phosphate buffer and 0.075M NaCl, pH 7.4.
Storage Conditions: Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
Physical Appearance: Filtered White lyophilized (freeze-dried) powder.
| Recombinant Human DAG1 General Information | |
|---|---|
| Alternate Names | |
| Dystroglycan, Dystrophin-associated glycoprotein 1, DAG1, A3a, DAG, AGRNR, 156DAG, MDDGC7, MDDGC9. | |
| Molecular Weight | |
| 31.87 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | Q14118 |
| General Description of Recombinant Human DAG1. | |
| Human DAG1 produced in E. Coli is a single, non-glycosylated, polypeptide chain (a. a 30-312) containing 293 amino acids including a 10 a. a N-terminal His tag. The total molecular mass is 31.87kDa (calculated). | |
Limitations and Performance Guarantee
enQuire Bio’s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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