PDF DatasheetDengue Virus Dengue Epitope 10 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant Dengue Epitope 10 based upon sequence from Dengue Virus
Host: QP11639 protein expressed in E. Coli.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of Dengue Epitope 10 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes
Other Applications: 1. Prepare 526-532nm colloid gold, conjugation within 24 hours after colloid gold preparation. 2. Adjust colloid gold OD at 1.3-1.4, conjugation concentration and pH are 16µg/ml gold and 7.2. 3. Shanking conjugation for 8-10 hours, then centrifuge the conjugation at 10,000 rpm at 20oC for 20 min. 4. Remove the supernatnant as more as possible, add the supplied gold suspension buffer to adjust OD to 8.2. 5. Add the supplied blocking reagent to the final concentration to 0.8%. 6. Blocking for 1 hour, then add sucrose to the final concentration to 10 to 12%. 7. Dipping the conjugated gold to the gold pad (do not use sodium phosphate for gold pad treatment). 8. After drying, assemble gold pad, the sample padand absorbent pad over backing card. 9. Add 10µl serum/plasma and add the supplied epi-10 running buffer for assay.
Bioactivity Data: Untested
Purity: Protein is >95% pure as determined by 12% PAGE (coomassie staining).
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: Phosphate buffered saline, pH 7.4 and 0.02% sodium nitrate.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Colorless Liquid
Limitations and Performance Guarantee
enQuire Bio’s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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