PDF Datasheetother DnaK Mycobacterium Tuberculosis Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant DnaK Mycobacterium Tuberculosis based upon sequence from other
Host: QP11686 protein expressed in E. Coli.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of DnaK Mycobacterium Tuberculosis was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized DnaK in sterile 18MΩ.cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Bioactivity Data: Untested
Amino Acid Sequence: MARAVGIDLG TTNSVVSVLE GGDPVVVANS EGSRTTPSTV AFARNGEVLV GQPAKNQAVT NVDRTIRSVK RHMGSDWSIE IDGKKYTAQE ISARVLMKLK RDAEAYLGED ITDAVITTPA YFNDAQRQAT KEAGQIAGLN VLRIVNEPTA AALAYGLDKG EREQTILVFD LGGGTFDVSL LEIGEGVVEV RATSGDNHLG GDDWDDRIVN WLVDKFKGTS GIDLTKDKMA MQRLREAAEK AKIELSSSQS TSVNLPYITV DSDKNPLFLD EQLIRAEFQR ITQDLLDRTR QPFQSVVKDA GISVSEIDHV VLVGGSTRMP AVTDLVKELT GGKEPNKGVN PDEVVAVGAA LQAGVLKGEV KDVLLLDVTP LSLGIETKGG VMTKLIERNT TIPTKRSETF TTADDNQPSV QIQVYQGERE IASHNKLLGS FELTGIPPAP RGVPQIEVTF DIDANGIVHV TAKDKGTGKE NTIKIQEGSG LSKEEIDRMV KDAEAHAEED RKRREEADVR NQAETLVYQT EKFVKEQRET ENGSRVPEDT LNKVEAAVAE AKTALGGTDI SAIKSAMEKL GQDSQALGQA IYEATQAASK VGGEASAPGG SNSTDDVVDA EVVDDERESK
Purity: Greater than 95.0% as determined by SDS-PAGE.
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: The DnaK protein was lyophilized from a concentrated (1 mg/ml) solution containing 10mM Na-phosphate buffer pH 7.4, 130mM NaCl and 2.5mM KCl.
Storage Conditions: Lyophilized DnaK although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution DnaK should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
| Recombinant other DnaK Mycobacterium Tuberculosis General Information | |
|---|---|
| Alternate Names | |
| HSP-70, HSP70, DnaK, Chaperone protein dnaK, Heat shock protein 70, Heat shock 70 kDa protein, HSP70, 70 kDa antigen, ML2496. | |
| Molecular Weight | |
| 66.7 kDa | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | dnaK |
| Entrez Gene ID | 908588 |
| UniProt ID(s) | P19993 |
| COSMIC ID Link(s) | dnaK |
| KEGG Gene ID(s) | mle:ML2496 |
| General Description of Recombinant other DnaK Mycobacterium Tuberculosis. | |
| Recombinant Mycobacterium Tuberculosis Dank produced in E. Coli is a single, non-glycosylated polypeptide chain containing 625 amino acids and having a molecular mass of 66.7 kDa. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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