PDF DatasheetHuman DNase Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant DNase based upon sequence from Human
Host: QP11692 protein expressed in CHO.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of DNase was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes
Definition of Unit (for specific activity): Dnase is generally assayed according to the photometric method developed by Kunitz. One Dnase unit results in an increase in absorbance at 260nm of 0.001/minute at 25°C when acting upon highly polymerized solution of DNA at pH 5. Also 0.005 Kunitz unit digests 1µgof lambda phage DNA in 10 minutes at 37°C in50mM Tris, 1mMMg++, pH 7.8 in a 50ul reaction.
Bioactivity Data:
Specific Activity: 1000IU/1 mg.
Amino Acid Sequence: MRGMKLLGAL LALAALLQGA VSLKIAAFNI QTFGETKMSN ATLVSYIVQI LSRYDIALVQ EVRDSHLTAV GKLLDNLNQD APDTYHYVVS EPLGRNSYKE RYLFVYRPDQ VSAVDSYYYD DGCEPCGNDT FNREPAIVRF FSRFTEVREF AIVPLHAAPG DAVAEIDALY DVYLDVQEKW GLEDVMLMGD FNAGCSYVRP SQWSSIRLWT SPTFQWLIPD SADTTATPTH CAYDRIVVAG MLLRGAVVPD SALPFNFQAA YGLSDQLAQA ISDHYPVEVM LK
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: Each mg contains 150 µg calcium chloride dehydrate and 8.77 mg NaCl.
Storage Conditions: 2 years when stored at 4°C, three weeks at 15°C, pH 6.3.
Physical Appearance: Sterile liquid colorless solution at a concentration of 1 mg/ml.
| Recombinant Human DNase General Information | |
|---|---|
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| Alternate Names | |
| EC 3.1. 21.1, Deoxyribonuclease I, DNase I, DNL1, DRNI, FLJ38093, DNASE1. | |
| Molecular Weight | |
| 37 kDa | |
| Chromosomal Location | |
| p13.3 on chromosome 16 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | DNASE1 |
| Entrez Gene ID | 1773 |
| UniProt ID(s) | P24855 |
| COSMIC ID Link(s) | DNASE1 |
| KEGG Gene ID(s) | hsa:1773 |
| General Description of Recombinant Human DNase. | |
| Human Deoxyribonuclease I produced in CHO is a glycosylated, polypeptide chain containing 260 amino acids and having a total molecular mass of 37,000 Dalton with a molecular formula of C1321H1995O396S9. DNase is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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