PDF DatasheetHuman EDN3 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant EDN3 based upon sequence from Human
Host: QP11747 protein expressed in E. Coli.
Available Tags: Untagged, His
Protein Construction: A cDNA sequence encoding the sequence of EDN3 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized EDN3 in sterile Acetic Acid not less than 0.5 mg/ml, which can then be further diluted to other aqueous solutions.
Bioactivity Data: Untested
Amino Acid Sequence: H-Cys-Thr-Cys-Phe-Thr-Tyr-Lys-Asp-Lys-Glu-Cys-Val-Tyr-Tyr-Cys-His-Leu-Asp-Ile-Ile-Trp-OH
Purity: Greater than 97.0% as determined by RP-HPLC.
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: The protein (1 mg/ml) was lyophilized with no additives.
Storage Conditions: Lyophilized EDN3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution EDN3 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
| Recombinant Human EDN3 General Information | |
|---|---|
| Alternate Names | |
| EDN3, EDN-3, ET-3, ET3, WS4B, HSCR4, MGC15067, MGC61498, Endothelin-3, Preproendothelin-3, PPET3. | |
| Molecular Weight | |
| 2.6 kDa | |
| Chromosomal Location | |
| q13.32 on chromosome 20 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | EDN3 |
| Entrez Gene ID | 1908 |
| UniProt ID(s) | P14138 |
| COSMIC ID Link(s) | EDN3 |
| KEGG Gene ID(s) | hsa:1908 |
| General Description of Recombinant Human EDN3. | |
| EDN3 contains 21 amino acids having a molecular mass of 2643.08 Dalton, and the molecular formula is: C121H168N26O33S4. Disulfide bonds between cys-1 and cys-15 + cys-3 and cys-11. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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