PDF DatasheetHuman EGFR Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant EGFR based upon sequence from Human
Host: QP11760 protein expressed in Insect, CHO.
Available Tags: Untagged, His
Protein Construction: A cDNA sequence encoding the sequence of EGFR was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized EGFR in sterile PBS not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Bioactivity Data: Untested
Amino Acid Sequence: LEEKKV CQGTSNKLTQ LGTFEDHFLS LQRMFNNCEV VLGNLEITYV QRNYDLSFLK TIQEVAGYVL IALNTVERIP LENLQIIRGN MYYENSYALA VLSNYDANKT GLKELPMRNL QEILHGAVRF SNNPALCNVE SIQWRDIVSS DFLSNMSMDF QNHLGSCQKC DPSCPNGSCW GAGEENCQKL TKIICAQQCS GRCRGKSPSD CCHNQCAAGC TGPRESDCLV CRKFRDEATC KDTCPPLMLY NPTTYQMDVN PEGKYSFGAT CVKKCPRNYV VTDHGSCVRA CGADSYEMEE DGVRKCKKCE GPCRKVCNGI GIGEFKDSLS INATNIKHFK NCTSISGDLH ILPVAFRGDS FTHTPPLDPQ ELDILKTVKE ITGFLLIQAW PENRTDLHAF ENLEIIRGRT KQHGQFSLAV VSLNITSLGL RSLKEISDGD VIISGNKNLC YANTINWKKL FGTSGQKTKI ISNRGENSCK ATGQVCHALC SPEGCWGPEP RDCVSCRNVS RGRECVDKCK LLEGEPREFV ENSECIQCHP ECLPQAMNIT CTGRGPDNCI QCAHYIDGPH CVKTCPAGVM GENNTLVWKY ADAGHVCHLC HPNCTYGCTG PGLEGCPTNG PKIPSIAASW SHPQFEK
Purity: Greater than 90.0% as determined by SDS-PAGE.
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: ErbB1 was lyophilized from a concentrated (1 mg/ml) sterile solution containing 1x PBS pH 7.4.
Storage Conditions: Lyophilized EGFR although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution EGFR should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
| Recombinant Human EGFR General Information | |
|---|---|
 |
|
| Alternate Names | |
| Epidermal growth factor receptor, EC 2.7. 10.1, Receptor tyrosine-protein kinase ErbB-1, ERBB, mENA, ERBB1, EGFR. | |
| Molecular Weight | |
| 134.3 kDa | |
| Chromosomal Location | |
| p11.2 on chromosome 7 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | EGFR |
| Entrez Gene ID | 1956 |
| UniProt ID(s) | P00533 |
| COSMIC ID Link(s) | EGFR |
| KEGG Gene ID(s) | hsa:1956 |
| General Description of Recombinant Human EGFR. | |
| The EGFR contains the extracellular domain of the human EGFR (25-647 AA ) excluding the signal peptide which is cleaved by the insect cells having an approximate MW of 85kDa. The EGFR is fused to a C-terminal Strep-tag and purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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![Analysis of Mass Spec data (dashed-line) of fractions stained with EGFR MS-QAVA™ monoclonal antibody [Clone: H9B4] (solid-line), reveals that less than 13.1% of signal is attributable to non-specific binding of anti-EGFR [Clone H9B4] to targets other than EGFR protein. Even frequently cited antibodies have much greater non-specific interactions, averaging over 30%. Data in image is from analysis in Jurkat, U202 and HeLa cells.](https://cdn-enquirebio.pressidium.com/wp-content/uploads/2017/10/enQuire-Bio-1956-MSM3-P1-anti-EGFR-antibody-150x150.png)
![Staining with mouse monoclonal EGFR [Clone 31G7] antibody in formalin-fixed paraffin-embedded human placenta.](https://enquirebio.com/wp-content/uploads/2019/01/EGFR IHC human Placenta (31G7)-150x150.jpg)
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