PDF DatasheetHuman FAM3D Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant FAM3D based upon sequence from Human
Host: QP11847 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of FAM3D was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes
Recommended Reconstitution Instructions: It is recommended to add 200µl of 0.1M Acetate buffer pH 4 to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely at 37°C. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10µg/ml. In higher concentrations the solubility of this antigen is limited. FAM3D is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Bioactivity Data: Untested
Amino Acid Sequence: MKHHHHHHASYMSFSMKTIR LPRWLAASPT KEIQVKKYKC GLIKPCPANY FAFKICSGAA NVVGPTMCFE DRMIMSPVKN NVGRGLNIAL VNGTTGAVLG QKAFDMYSGD VMHLVKFLKE IPGGALVLVA SYDDPGTKMN DESRKLFSDL GSSYAKQLGF RDSWVFIGAK DLRGKSPFEQ FLKNSPDTNK YEGWPELLEM EGCMPPKPF
Purity: Greater than 90.0% as determined by SDS-PAGE.
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: FAM3D filtered (0.4 µm) and lyophilized from 0.5 mg/ml in 0.05M Acetate buffer pH 4.0.
Storage Conditions: Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
Physical Appearance: Filtered White lyophilized (freeze-dried) powder.
| Recombinant Human FAM3D General Information | |
|---|---|
| Alternate Names | |
| Protein FAM3D, FAM3D, EF7, OIT1. | |
| Molecular Weight | |
| 23.3 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | Q96BQ1 |
| General Description of Recombinant Human FAM3D. | |
| Human FAM3D produced in E. Coli is a single, non-glycosylated, polypeptide chain (a. a 26-224) containing 209 amino acids including a 10 a. a N-terminal His tag. The total molecular mass is 23.3kDa (calculated). | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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