PDF DatasheetHuman FETUB Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant FETUB based upon sequence from Human
Host: QP11868 protein expressed in HEK 293.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of FETUB was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes
Recommended Reconstitution Instructions: It is recommended to add deionized water to a working concentration of 0.5 mg/ml and let the lyophilized pellet dissolve completely. FETUB is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Bioactivity Data: Untested
Amino Acid Sequence: HSHVCGAMSP PQLALNPSAL LSRGCNDSDV LAVAGFALRD INKDRKDGYV LRLNRVNDAQ EYRRGGLGSL FYLTLDVLET DCHVLRKKAW QDCGMRIFFE SVYGQCKAIF YMNNPSRVLY LAAYNCTLRP VSKKKIYMTC PDCPSSIPTD SSNHQVLEAA TESLAKYNNE NTSKQYSLFK VTRASSQWVV GPSYFVEYLI KESPCTKSQA SSCSLQSSDS VPVGLCKGSL TRTHWEKFVS VTCDFFESQA PATGSENSAV NQKPTNLPKV EESQQKNTPP TDSPSKAGPR GSVQYLPDLD DKNSQEKGPQ EAFPVHLDLT TNPQGETLDI SFLFLEPMEE KLVVLPFPKE KARTAECPGP AQNASPLVLP PHHHHHH
Purity: Greater than 90.0% as determined by SDS-PAGE.
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: FETUB filtered (0.4µm) and lyophilized from 0.5 mg/ml in 0.05M phosphate buffer and 0.075M NaCl, pH 7.4.
Storage Conditions: Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
Physical Appearance: Filtered White lyophilized (freeze-dried) powder.
| Recombinant Human FETUB General Information | |
|---|---|
| Alternate Names | |
| Fetuin-B, 16G2, Fetuin-like protein IRL685, Gugu, FETUB, IRL685. | |
| Molecular Weight | |
| 41.8 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | Q9UGM5 |
| General Description of Recombinant Human FETUB. | |
| Human Fetuin-B produced in HEK cells is a single, glycosylated, polypeptide chain containing a total of 377 amino acids, having a molecular mass of 41.8kDa (calculated) though it migrates at approximately 55kDa on SDS PAGE, the Fetuin-B is also composed of a 4 a. a N-terminal linker and fused to a 6 a. a His tag at C-Terminus. The Human FETUB is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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