PDF DatasheetHuman Fibronectin Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant Fibronectin based upon sequence from Human
Host: QP11881 protein expressed in Native Protein.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of Fibronectin was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes
Recommended Reconstitution Instructions: Purified fibronectin has a tendency to form insoluble aggregates upon reconstitution. We suggest reconstituting the 1 mg Fibronectin with a chaotropic agent such as urea at room temperature at a concentration of 0.2 mg/ml using sterile water. Let stand 1-2 hours. The recommended concentration is 4M-5M urea. When using the protein as an attachment factor, wash the urea off after attaching the fibronectin to the growth surface (plate or dish).
Bioactivity Data: Untested
Purity: Greater than 95.0% as determined by SDS-PAGE.
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: The Fibronectin was lyophilized from a concentrated 1 mg/ml solution containing 0.05M Tris Cl pH 7.5 and 0.1M NaCl.
Storage Conditions: Store the lyophilized fibronectin at 4°C. Upon reconstitution fibronectin should be stored at 4°C for 2 weeks and for future use below -18°C. Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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