PDF DatasheetHuman GAD2 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant GAD2 based upon sequence from Human
Host: QP11919 protein expressed in Insect.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of GAD2 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes
Other Applications: 1. Binds IgG type human auto-antibodies. 2. Standard ELISA test (checker-board analysis of positive/negative sera panels).
Bioactivity Data: Untested
Amino Acid Sequence: MASPGSGFWS FGSEDGSGDS ENPGTARAWC QVAQKFTGGI GNKLCALLYG DAEKPAESGG SQPPRAAARK AACACDQKPC SCSKVDVNYA FLHATDLLPA CDGERPTLAF LQDVMNILLQ YVVKSFDRST KVIDFHYPNE LLQEYNWELA DQPQNLEEIL MHCQTTLKYA IKTGHPRYFN QLSTGLDMVG LAADWLTSTA NTNMFTYEIA PVFVLLEYVT LKKMREIIGW PGGSGDGIFS PGGAISNMYA MMIARFKMFP EVKEKGMAAL PRLIAFTSEH SHFSLKKGAA ALGIGTDSVI LIKCDERGKM IPSDLERRIL EAKQKGFVPF LVSATAGTTV YGAFDPLLAV ADICKKYKIW MHVDAAWGGG LLMSRKHKWK LSGVERANSV TWNPHKMMGV PLQCSALLVR EEGLMQNCNQ MHASYLFQQD KHYDLSYDTG DKALQCGRHV DVFKLWLMWR AKGTTGFEAH VDKCLELAEY LYNIIKNREG YEMVFDGKPQ HTNVCFWYIP PSLRTLEDNE ERMSRLSKVA PVIKARMMEY GTTMVSYQPL GDKVNFFRMV ISNPAATHQD IDFLIEEIER LGQDL
Purity: Greater than 95.0% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: GAD2 protein solution is supplied in 16mM HEPES buffer pH 7.5, 250mM NaCl, 0.016mM Pyridoxal-5-Phosphate, 0.16% Triton x-100 and 20% glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered clear solution.
| Recombinant Human GAD2 General Information | |
|---|---|
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| Alternate Names | |
| Glutamate Decarboxylase 2 (Pancreatic Islets And Brain, 65kDa), Glutamate Decarboxylase 65 KDa Isoform, 65 KDa Glutamic Acid Decarboxylase, EC 4.1. 1.15, GAD-65, GAD65, Glutamate Decarboxylase 2 (Pancreatic Islets And Brain, 65kD), Glutamate Decarboxylase-2 (Pancreas), EC 4.1. 1, GAD2. | |
| Molecular Weight | |
| 66.2 kDa | |
| Chromosomal Location | |
| p12.1 on chromosome 10 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | GAD2 |
| Entrez Gene ID | 2572 |
| UniProt ID(s) | Q05329 |
| COSMIC ID Link(s) | GAD2 |
| KEGG Gene ID(s) | hsa:2572 |
| General Description of Recombinant Human GAD2. | |
| Recombinant Human GAD2 produced in SF9 is a glycosylated, polypeptide chain having a calculated molecular mass of 66,234 Dalton. GAD2 is expressed with a 6xHis tag at N-terminus and purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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