PDF DatasheetHuman GDF3 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant GDF3 based upon sequence from Human
Host: QP11950 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of GDF3 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized GDF3 in sterile 100mM Acetate buffer pH 4 at a concentration of 0.5 mg/ml. For the dilution into higher pH values, it is recommended to dilute the protein to a concentration of 10?g/ml. Please note that in higher concentrations the solubility of GDF3 is limited. The protein is not sterile! Please sterile filter before using it in the cell culture.
Bioactivity Data: Untested
Amino Acid Sequence: MKHHHHHHAS AAIPVPKLSC KNLCHRHQLF INFRDLGWHK WIIAPKGFMA NYCHGECPFS LTISLNSSNY AFMQALMHAV DPEIPQAVCI PTKLSPISML YQDNNDNVIL RHYEDMVVDECGCG
Purity: Greater than 90.0% as determined by SDS-PAGE.
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: The protein was lyophilized from a concentrated solution (0.5 mg/ml) containing 30mM Acetate buffer pH 4.
Storage Conditions: Lyophilized GDF3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution GDF3 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance: Filtered White lyophilized (freeze-dried) powder.
| Recombinant Human GDF3 General Information | |
|---|---|
| Alternate Names | |
| Growth Differentiation Factor 3, Growth/Differentiation Factor 3 , MCOPCB6, MCOP7, GDF-3, KFS3. | |
| Molecular Weight | |
| 14.15 kDa | |
| Chromosomal Location | |
| p13.31 on chromosome 12 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | GDF3 |
| Entrez Gene ID | 9573 |
| UniProt ID(s) | Q9NR23 |
| COSMIC ID Link(s) | GDF3 |
| KEGG Gene ID(s) | hsa:9573 |
| General Description of Recombinant Human GDF3. | |
| Human GDF3 produced in E. Coli is a single, non-glycosylated, polypeptide chain containing 124 amino acids and having a total molecular mass of 14.15 kDa. GDF3 is fused to a 10 amino acid His Tag at N-terminus and purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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