PDF DatasheetOvine GHBP Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant GHBP based upon sequence from Ovine
Host: QP11966 protein expressed in E. Coli.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of GHBP was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized Growth Hormone Binding Protein in sterile 18MΩ.cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Bioactivity Data: Untested
Amino Acid Sequence: MDLWQLLLTL AVAGSSDAFS GSEATPAFFV RASQSLQILY PGLETNSSGN LKFTKCRSPE LETFSCHWTD GANHSLQSPG SVQMFYIRRD IQEWKECPDY VSAGENSCYF NSSYTSVWTP YCIKLTSNGG IVDHKCFSVE DIVQPDPPVG LNWTLLNISL TEIHADILVK WEPPPNTDVK MGWIILEYEL HYKELNETQW KMMDPLLVTS VPMYSLRLDK EYEVRVRTRQ RNTEKYGKFS EVLLITFPQM NPSACEEDFQ FPWFLIIIFG ILGLTVTLFL LIFSKQQRIK MLILPPVPVP KIKGIDPDLL KEGKLEEVNT ILAIHDNYKH EFYNDDSWVE FIELDIDDPD EKTEGSDTDR LLSNDHEKSL SIFGAKDDDS GRTSCYEPDI LETDFHVSDM CDGTSEVAQP QRLKGEADIL CLDQKNQNNS PSNDAAPASQ QPSVILVEEN KPRPLPIGGT ESTHQAVHTQ LSNPSSLANI DFYAQVSDIT PAGNVVLSPG QKNKTGNPQC DTHPEVVTPS QADFIVDSAY FCEVDAKKYI ALAPDVEAES HIEPSFNQED IYITTESLTT TAGRSGTAEN VPSSEIPVPD YTSIHIVQSP QGLVLNATAL PLPDKEFLSS CGYVSTDQLN KIMP
Purity: Greater than 95.0% as determined by:(a) Analysis by SEC-HPLC. (b) Analysis by SDS-PAGE.
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: GHBP was lyophilized from a concentrated (1 mg/ml) solution with 0.0045mM NaHCO3.
Storage Conditions: Lyophilized Growth Hormone Binding Protein although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution GHBP should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
| Recombinant Ovine GHBP General Information | |
|---|---|
| Alternate Names | |
| GHR, GHBP, GH receptor, Somatotropin receptor. | |
| Molecular Weight | |
| 28 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | Q28575 |
| General Description of Recombinant Ovine GHBP. | |
| Growth Hormone Binding Protein Ovine Recombinant produced in E. Coli is a single, non-glycosylated, polypeptide chain containing 237 amino acids and having a molecular mass of 28 kDa. GHBP is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs,agricultural or pesticidal products, food additives or household chemicals. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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