PDF Datasheetother Gly m 5.0101 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant Gly m 5.0101 based upon sequence from other
Host: QP11998 protein expressed in Insect.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of Gly m 5.0101 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes
Other Applications: 1. Binds IgE type human antibodies. 2. Immunodot test with positive/negative sera panels.
Bioactivity Data: Untested
Amino Acid Sequence: VEKEECEEGE IPRPRPRPQH PEREPQQPGE KEEDEDEQPR PIPFPRPQPR QEEEHEQREE QEWPRKEEKR GEKGSEEEDE DEDEEQDERQ FPFPRPPHQK EERKQEEDED EEQQRESEES EDSELRRHKN KNPFLFGSNR FETLFKNQYG RIRVLQRFNQ RSPQLQNLRD YRILEFNSKP NTLLLPNHAD ADYLIVILNG TAILSLVNND DRDSYRLQSG DALRVPSGTT YYVVNPDNNE NLRLITLAIP VNKPGRFESF FLSSTEAQQS YLQGFSRNIL EASYDTKFEE INKVLFSREE GQQQGEQRLQ ESVIVEISKE QIRALSKRAK SSSRKTISSE DKPFNLRSRD PIYSNKLGKF FEITPEKNPQ LRDLDIFLSI VDMNEGALLL PHFNSKAIVI LVINEGDANI ELVGLKEQQQ EQQQEEQPLE VRKYRAELSE QDIFVIPAGY PVVVNATSNL NFFAIGINAE NNQRNFLAGS QDNVISQIPS QVQELAFPGS AQAVEKLLKN QRESYFVDAQ PKKKEEGNKG RKGPLSSILR AFY
Purity: Greater than 80.0% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: Gly m 5.0101 is supplied in 20mM HEPES buffer pH 7.6, 250mM NaCl and 20% glycerol.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered clear solution.
| Recombinant other Gly m 5.0101 General Information | |
|---|---|
| Alternate Names | |
| Alpha subunit of beta conglycinin, Gly m 5. | |
| Molecular Weight | |
| 70.6 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | O22120 |
| General Description of Recombinant other Gly m 5.0101. | |
| Recombinant Alpha Subunit of Beta Conglycinin produced in SF9 is a glycosylated, polypeptide chain having a calculated molecular mass of 70,586 Dalton. Gly m 5.0101 is expressed with a 10xHis tag at N-terminus and purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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