PDF DatasheetHuman GPNMB Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant GPNMB based upon sequence from Human
Host: QP12041 protein expressed in HEK 293, E. Coli, Insect.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of GPNMB was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes
Recommended Reconstitution Instructions: It is recommended to add deionized water to a working concentration of 0.5 mg/ml and let the lyophilized pellet dissolve completely. GPNMB is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Bioactivity Data: Untested
Amino Acid Sequence: AKRFHDVLGN ERPSAYMREH NQLNGWSSDE NDWNEKLYPV WKRGDMRWKN SWKGGRVQAV LTSDSPALVG SNITFAVNLI FPRCQKEDAN GNIVYEKNCR NEAGLSADPY VYNWTAWSED SDGENGTGQS HHNVFPDGKP FPHHPGWRRW NFIYVFHTLG QYFQKLGRCS VRVSVNTANV TLGPQLMEVT VYRRHGRAYV PIAQVKDVYV VTDQIPVFVT MFQKNDRNSS DETFLKDLPI MFDVLIHDPS HFLNYSTINY KWSFGDNTGL FVSTNHTVNH TYVLNGTFSL NLTVKAAAPG PCPPPPPPPR PSKPTPSLAT TLKSYDSNTP GPAGDNPLEL SRIPDENCQI NRYGHFQATI TIVEGILEVN IIQMTDVLMP VPWPESSLID FVVTCQGSIP TEVCTIISDP TCEITQNTVC SPVDVDEMCL LTVRRTFNGS GTYCVNLTLG DDTSLALTST LISVP KLHHH HHHHHHH
Purity: Greater than 95.0% as determined by SDS-PAGE.
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: Filtered (0.4µm) and lyophilized from 0.5 mg/ml in 0.05M phosphate buffer and 0.075M NaCl, pH 7.4.
Storage Conditions: Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time.
Physical Appearance: Filtered White lyophilized (freeze-dried) powder.
| Recombinant Human GPNMB General Information | |
|---|---|
| Alternate Names | |
| Transmembrane glycoprotein NMB, Transmembrane glycoprotein HGFIN, GPNMB, HGFIN, NMB. | |
| Molecular Weight | |
| 53.7 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | Q14956 |
| General Description of Recombinant Human GPNMB. | |
| Human GPNMB produced in HEK cells is a single, glycosylated, polypeptide chain (a. a 22-486) containing a total of 477 amino acids, having a molecular mass of 53.7kDa (calculated), though it migrates at approximately 97kDa on SDS PAGE, the GPNMB is fused to a 2 a. a C-terminal linker and a 10 a. a His tag at C-Terminus. The Human GPNMB is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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