PDF DatasheetHIV HIV-1 gp120 LAV Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant HIV-1 gp120 LAV based upon sequence from HIV
Host: QP12245 protein expressed in Insect.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of HIV-1 gp120 LAV was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes
Additional Testing: Confirmed to react with antibodies from sera of from HIV infected individuals. Western blots: 0.1-1.0 µg/strip.
Bioactivity Data: Untested
Amino Acid Sequence: IPGEKLWVTV YYGVPVWKEA TTTLFCASDA KAYDTEVHNV ATHACVPTDP NPQEVVLVNV TENFNMWKND MVEQMHEDII SLWDQSLKPC VKLTPLCVSL KCTDLKNDTN TNSSSGRMIM EKGEIKNCSF NISTSIRDKV QKEYAFFYKL DIVPIDNTSY RLISCNTSVI TQACPKVSFE PIPIHYCAPA GFAILKCNNK TFNGTGPCTN VSTVQCTHGI RPVVSTQLLL NGSLAEEDVV IRSANFTDNA KTIIVQLNTS VEINCTRPNN NTRKSIRIQR GPGRAFVTIG KIGNMRQAHC NISRAKWNAT LKQIASKLRE QFGNNKTIIF KQSSGGDPEI VTHSFNCGGE FFYCNSTQLF NSTWFNSTWS TEGSNNTEGS DTITLPCRIK QFINMWQEVG KAMYAPPISG QIRCSSNITG LLLTRDGGNN NNGSEIFRPG GGDMRDNWRS ELYKYKVVKI EPLGVAPTKA KRRVVQREKR
Purity: Greater than 90.0% as determined by HPLC analysis and SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: The protein solution contains 10mM Tris-Cl pH 7.6, 150mM NaCl and 0.01% Triton N-101.
Storage Conditions: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile filtered clear solution.
| Recombinant HIV HIV-1 gp120 LAV General Information | |
|---|---|
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | M19921 |
| COSMIC ID Link(s) | – |
| General Description of Recombinant HIV HIV-1 gp120 LAV. | |
| HIV-1 gp120 LAV isolate Recombinant- is the external envelope protein, full-length 100-120 kDa, derived from the env. gene of HIV-1 and glycosylated with N-linked sugars and produced using Baculovirus vectors in insect cells. Purified under conditions that maintain the tertiary structure of the biologically active molecule. HIV-1 gp120 LAV sequence is identical to the predicted amino acid sequence of gp120 from pNL4-3 (Adachi et al. [1986], J. Viral. 59.284-291; GenBank accession number M19921). | |
Limitations and Performance Guarantee
enQuire Bio’s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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