PDF DatasheetHIV HIV-1 nef, Clade B Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant HIV-1 nef, Clade B based upon sequence from HIV
Host: QP12254 protein expressed in E. Coli.
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of HIV-1 nef, Clade B was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized HIV-1 nef in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Bioactivity Data: Untested
Purity: Greater than 90.0% as determined by HPLC analysis and SDS-PAGE.
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: Lyophilized with 10% glycerol.
Storage Conditions: Lyophilized HIV-1 nef although stable at room temperature for 1 week, should be stored desiccated below -18°C. Upon reconstitution HIV-1 nef should be stored at 4°C between 2-7 days and for future use below -18°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered and lyophilized, though might appear as a solution as a result of the glycerol content.
| Recombinant HIV HIV-1 nef, Clade B General Information | |
|---|---|
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | CAA41585 |
| COSMIC ID Link(s) | – |
| General Description of Recombinant HIV HIV-1 nef, Clade B. | |
| The E. coli derived 27 kDa recombinant HIV-1 Nef Clade-B protein is a single non-glycosylated polypeptide chain. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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