PDF DatasheetHuman HSPA1B Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant HSPA1B based upon sequence from Human
Host: QP12328 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of HSPA1B was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes
Recommended Reconstitution Instructions: It is recommended to add deionized water to prepare a working stock solution of 0.5 mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Bioactivity Data: Untested
Amino Acid Sequence: MKHHHHHHAS AKAAAIGIDL GTTYSCVGVF QHGKVEIIAN DQGNRTTPSY VAFTDTERLI GDAAKNQVAL NPQNTVFDAK RLIGRKFGDP VVQSDMKHWP FQVINDGDKP KVQVSYKGET KAFYPEEISS MVLTKMKEIA EAYLGYPVTN AVITVPAYFN DSQRQATKDA GVIAGLNVLR IINEPTAAAI AYGLDRTGKG ERNVLIFDLG GGTFDVSILT IDDGIFEVKA TAGDTHLGGEDFDNRLVNHF VEEFKRKHKK DISQNKRAVR RLRTACERAK RTLSSSTQAS LEIDSLFEGI DFYTSITRAR FEELCSDLFR STLEPVEKAL RDAKLDKAQI HDLVLVGGST RIPKVQKLLQ DFFNGRDLNK SINPDEAVAY GAAVQAAILM GDKSENVQDL LLLDVAPLSL GLETAGGVMT ALIKRNSTIP TKQTQIFTTY SDNQPGVLIQ VYEGERAMTK DNNLLGRFEL SGIPPAPRGVPQIEVTFDID ANGILNVTAT DKSTGKANKI TITNDKGRLS KEEIERMVQE AEKYKAEDEV QRERVSAKNA LESYAFNMKS AVEDEGLKGK ISEADKKKVL DKCQEVISWL DANTLAEKDE FEHKRKELEQ VCNPIISGLY QGAGGPGPGG FGAQGPKGGS GSGPTIEEVD
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: The protein was filtered (0.4µm) and lyophilized from a 0.5 mg/ml solution containing 50mM phosphate buffer, 75mM NaCl, pH 7.4.
Storage Conditions: Lyophilized HSPA1B although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution HSPA1B should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
| Recombinant Human HSPA1B General Information | |
|---|---|
| Alternate Names | |
| Heat shock 70kDa protein 1B, HSP70-2, HSP70-1/HSP70-2, FLJ54328, HSPA1A, heat shock 70kDa protein 1A/1B, HSPA1. | |
| Molecular Weight | |
| 71.16 kDa | |
| Curated Database and Bioinformatic Data | |
| UniProt ID(s) | P0DMV9 |
| General Description of Recombinant Human HSPA1B. | |
| The HSPA1B Protein Human produced in E. Coli is a single, non-glycosylated polypeptide chain containing 650 amino acids having a molecular mass of 71.16kDa. HSPA1B is fused to a 10 amino acids His-Tag at N-terminus and purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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