PDF DatasheetHuman HSPB8 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant HSPB8 based upon sequence from Human
Host: QP12335 protein expressed in E. Coli.
Available Tags: Untagged, His
Protein Construction: A cDNA sequence encoding the sequence of HSPB8 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized HSPB8 in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Additional Validation: Immunoreactivity is confirmed by reaction with monoclonal mouse antibodies against HSPB8.
Bioactivity Data: Untested
Amino Acid Sequence: MADGQMPFSC HYPSRLRRDP FRDSPLSSRL LDDGFGMDPF PDDLTASWPD WALPRLSSAW PGTLRSGMVP RGPTATARFG VPAEGRTPPP FPGEPWKVCV NVHSFKPEEL MVKTKDGYVE VSGKHEEKQQ EGGIVSKNFT KKIQLPAEVD PVTVFASLSP EGLLIIEAPQ VPPYSTFGES SFNNELPQDS QEVTCT
Purity: Greater than 95.0% as determined by SDS-PAGE.
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: The HSPB8 protein was lyophilized from a concentrated (1 mg/ml) solution containing 20mM Tris-acetate, pH 7.6, 10mM NaCl, 0.1mM EDTA, 0.1mM PMSF, 15mM b-ME.
Storage Conditions: Lyophilized HSPB8 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution HSPB8 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
| Recombinant Human HSPB8 General Information | |
|---|---|
| Alternate Names | |
| HSPB8, H11, HMN2, CMT2L, DHMN2, E2IG1, HMN2A, HSP22, Heat shock protein beta-8, Alpha-crystallin C chain, Small stress protein-like protein HSP22, E2-induced gene 1 protein, Protein kinase H11, CRYAC. | |
| Molecular Weight | |
| 21.6 kDa | |
| Chromosomal Location | |
| q24.23 on chromosome 12 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | HSPB8 |
| Entrez Gene ID | 26353 |
| UniProt ID(s) | Q9UJY1 |
| COSMIC ID Link(s) | HSPB8 |
| KEGG Gene ID(s) | hsa:26353 |
| General Description of Recombinant Human HSPB8. | |
| Recombinant Human Heat Shock Protein 22 kDa Protein-8 is a full-length human HSP22 with an MW of 21604 Dalton produced in E. coli. | |
Selected References
Roles of ERK and NF-?B in Interleukin-8 Expression in Response to Heat Shock Protein 22 in Vascular Smooth Muscle Cells. Publication: Korean J Physiol Pharmacol. 2008 Aug;12(4):171-176.Link: http://synapse.koreamed.org/DOIx.php?id=10.4196/kjpp.2008.12.4.171&vmode=FULLApplications: HSP22 induces IL-8 in VSMCs via ERK1/2.
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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