PDF DatasheetBovine HTF Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant HTF based upon sequence from Bovine
Available Tags: Untagged
Protein Construction: A cDNA sequence encoding the sequence of HTF was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes Bovine Transferrin is a crucial component for the cultivation of mammalian cells in-vitro. Bovine Transferrin is Critical for long-term cells growth in-vitro. Bovine Transferrin is used as detoxificant in media by binding contaminating metal ions. Bovine Transferrin is often used as a nutrient in fermentation media for recombinant protein and biopharmaceutical production. Additional common uses of Bovine Transferrin areMolecular weight, Affinity purification of anti-human transferrin antibodies and also as receptor mediated transfection of molecules such as DNA, into cells.
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized Bovine HTF in sterile 18MΩ.cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Bioactivity Data: Untested
Amino Acid Sequence: MRPAVRALLA CAVLGLCLAD PERTVRWCTI STHEANKCAS FRENVLRILE SGPFVSCVKK TSHMDCIKAI SNNEADAVTL DGGLVYEAGL KPNNLKPVVA EFHGTKDNPQ THYYAVAVVK KDTDFKLNEL RGKKSCHTGL GRSAGWNIPM AKLYKELPDP QESIQRAAAN FFSASCVPCA DQSSFPKLCQ LCAGKGTDKC ACSNHEPYFG YSGAFKCLME GAGDVAFVKH STVFDNLPNP EDRKNYELLC GDNTRKSVDD YQECYLAMVP SHAVVARTVG GKEDVIWELL NHAQEHFGKD KPDNFQLFQS PHGKDLLFKD SADGFLKIPS KMDFELYLGY EYVTALQNLR ESKPPDSSKD ECMVKWCAIG HQERTKCDRW SGFSGGAIEC ETAENTEECI AKIMKGEADA MSLDGGYLYI AGKCGLVPVL AENYKTEGES CKNTPEKGYL AVAVVKTSDA NINWNNLKDK KSCHTAVDRT AGWNIPMGLL YSKINNCKFD EFFSAGCAPG SPRNSSLCAL CIGSEKGTGK ECVPNSNERY YGYTGAFRCL VEKGDVAFVK DQTVIQNTDG NNNEAWAKNL KKENFEVLCK DGTRKPVTDA ENCHLARGPN HAVVSRKDKA TCVEKILNKQ QDDFGKSVTD CTSNFCLFQS NSKDLLFRDD TKCLASIAKK TYDSYLGDDY VRAMTNLRQC STSKLLEACT FHKP
Purity: Greater than 97.0% as determined by Cellulose Acetate.
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: The protein (1 mg/ml) was lyophilized with no additives.
Storage Conditions: Lyophilized Bovine HTF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Bovine HTF should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered Pink lyophilized (freeze-dried) powder.
| Recombinant Bovine HTF General Information | |
|---|---|
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| Alternate Names | |
| Serotransferrin, Transferrin, Siderophilin, Beta-1-metal-binding globulin, TF, PRO1557, PRO2086, DKFZp781D0156, Holo Transferrin, HTF. | |
| Molecular Weight | |
| 77.8 kDa | |
| Chromosomal Location | |
| On chromosome 1 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | TF |
| Entrez Gene ID | 280705 |
| UniProt ID(s) | Q29443 |
| COSMIC ID Link(s) | TF |
| KEGG Gene ID(s) | bta:280705 |
| General Description of Recombinant Bovine HTF. | |
| Bovine Holo Transferrin is a glycoprotein of approximately 80 kDa. | |
Limitations and Performance Guarantee
enQuire Bio’s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. FDA approved Plasma from each donor has been tested and found negative for antibody to HIV-1, HIV-2, HCV, HBSAG, HBc, ALT and Syphilis. Viral inactivation by pasteurization (60°C for 10 hours) has been validated using three different test viruses, with removal of 8-14.5 logs of virus documented. The purification process has also been found to remove significant additional quantities of virus. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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