PDF DatasheetHuman IGFBP 6 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant IGFBP 6 based upon sequence from Human
Host: QP12381 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of IGFBP 6 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized Insulin-Like Growth Factor Binding Protein-6 in sterile 18MΩ.cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Bioactivity Data: Untested
Amino Acid Sequence: MTPHRLLPPL LLLLALLLAA SPGGALARCP GCGQGVQAGC PGGCVEEEDG GSPAEGCAEA EGCLRREGQE CGVYTPNCAP GLQCHPPKDD EAPLRALLLG RGRCLPARAP AVAEENPKES KPQAGTARPQ DVNRRDQQRN PGTSTTPSQP NSAGVQDTEM GPCRRHLDSV LQQLQTEVYR GAQTLYVPNC DHRGFYRKRQ CRSSQGQRRG PCWCVDRMGK SLPGSPDGNG SSSCPTGSSG
Purity: Greater than 80.0% as determined by SDS-PAGE.
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: IBP-6 was lyophilized from a concentrated (1 mg/ml) solution containing 1xPBS, 0.1% SDS and 1mM DTT.
Storage Conditions: Lyophilized IBP6 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IGFBP-6 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
| Recombinant Human IGFBP 6 General Information | |
|---|---|
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| Alternate Names | |
| IGFBP-6, IBP-6, IGF-binding protein 6. | |
| Molecular Weight | |
| 20 kDa | |
| Chromosomal Location | |
| q13.13 on chromosome 12 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | IGFBP6 |
| Entrez Gene ID | 3489 |
| UniProt ID(s) | P24592 |
| COSMIC ID Link(s) | IGFBP6 |
| KEGG Gene ID(s) | hsa:3489 |
| General Description of Recombinant Human IGFBP 6. | |
| Human IGFBP6 produced in E. Coli is a single, non-glycosylated, polypeptide chain containing amino acids 148-240 and having a molecular mass of 20 kDa including 4kDa His tag. IGFBP6 is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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