PDF Datasheetother Internalin-A Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant Internalin-A based upon sequence from other
Host: QP12452 protein expressed in E. Coli.
Available Tags: His
Protein Construction: A cDNA sequence encoding the sequence of Internalin-A was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes
Recommended Reconstitution Instructions: It is recommended to reconstitute the lyophilized Internalin-A in sterile 2XPBS not less than 100µg/ml, which can then be further diluted to other aqueous solutions. Important note: If the concentration of NaCl is less than 150mM, the protein solution becomes turbid overnight. Please always keep the concentration of NaCl more than 250mM.
Bioactivity Data: Untested
Amino Acid Sequence: MANENNFLEI MSETKEIKIV NIPDNNLKKV LNKSLNKSEN SDLTVKDLES IEYLYGIAEN ISNIEGLEYC KNLKILSLQN NDNSKKENFN TITDLSPLKY LKNLVVLDLR NNKISDLSPL ENLTNLESLR LSGNNISNIS PLNKLESLTT LTLSYNEITD ISTISNLKNL THLALYNNKI EDISSLKENT KLQNLSLGFN KIKDISVLSN LKNLYDLSLE ENNIKSIKSL SNLHKLSNIN LKNNKIEDIS PLETCNDFFE KLILDGNRIS DISSLSKKEI IVCSINNQTI TIKDWKVSDN NSFEIKLPND NLDIKIDNIS ENGTFIDNSI KWTNISPKSK LSFEFKQDID NDIHSIFYSG TVRFKRLEHHHH HH
Purity: Greater than 95.0% as determined by SDS-PAGE and HPLC.
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: Internalin-A is lyophilized from a 0.2µm filtered concentrated solution in 20mM PB pH 7.4 and 300mM NaCl.
Storage Conditions: Lyophilized Internalin-A although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Internalin-A should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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