PDF DatasheetHuman MAPK1 Recombinant Protein Product Attributes
Product Type: Recombinant Protein
Recombinant MAPK1 based upon sequence from Human
Host: QP12639 protein expressed in E. Coli.
Available Tags: Untagged, His
Protein Construction: A cDNA sequence encoding the sequence of MAPK1 was constructred and used to recombinantly synthesize the protein.
Recommended Applications: See Application Notes
Application Notes
Definition of Unit (for specific activity): 1 U = 1 pmol/min transferred to myelin basic protein at 30°C.
Bioactivity Data: Untested
Amino Acid Sequence: MAAAAAAGAG PEMVRGQVFD VGPRYTNLSY IGEGAYGMVC SAYDNVNKVR VAIKKISPFE HQTYCQRTLR EIKILLRFRH ENIIGINDII RAPTIEQMKD VYIVQDLMET DLYKLLKTQH LSNDHICYFL YQILRGLKYI HSANVLHRDL KPSNLLLNTT CDLKICDFGL ARVADPDHDH TGFLTEYVAT RWYRAPEIML NSKGYTKSID IWSVGCILAE MLSNRPIFPG KHYLDQLNHI LGILGSPSQE DLNCIINLKA RNYLLSLPHK NKVPWNRLFP NADSKALDLL DKMLTFNPHK RIEVEQALAH PYLEQYYDPS DEPIAEAPFK FDMELDDLPK EKLKELIFEE TARFQPGYRS
Purity: Greater than 95.0% as determined by SDS-PAGE.
Reconstitution Instructions: |||
Endotoxin Levels: Not Determined. Testing recommended prior to use in cell culture and can be performed upon request.
Buffer: MAPK1 is supplied containing 50mM Tris-HCL, 150mM NaCl, 2mM DTT, pH 8.0, 100 Units*/mg.
Storage Conditions: MAPK1 should be stored at 4°C if entire vial will be used within 2-4 weeks. For long term storage it is recommended to store at -20°C. Avoid multiple freeze-thaw cycles.
Physical Appearance: Sterile Filtered clear solution.
| Recombinant Human MAPK1 General Information | |
|---|---|
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| Alternate Names | |
| Mitogen-activated protein kinase 1, EC 2.7. 11.24, Extracellular signal-regulated kinase 2, ERK-2, Mitogen-activated protein kinase 2, MAP kinase 2, MAPK 2, p42-MAPK, ERT1, ERK, p38, p40, p41, ERK2, MAPK2, PRKM1, PRKM2, P42MAPK, p41mapk. | |
| Molecular Weight | |
| 44.6 kDa | |
| Chromosomal Location | |
| q11.22 on chromosome 22 | |
| Curated Database and Bioinformatic Data | |
| Gene Symbol | MAPK1 |
| Entrez Gene ID | 5594 |
| UniProt ID(s) | P28482 |
| COSMIC ID Link(s) | MAPK1 |
| KEGG Gene ID(s) | hsa:5594 |
| General Description of Recombinant Human MAPK1. | |
| MAPK1 Recombinant (extracellular signal-regulated kinase) a Mitogen-Activated Protein Kinase, is a highly active form produced by phosphorylation of the purified ERK2/MAPK1 in vitro with MEK1 is a non-glycosylated polypeptide having a molecular mass of 44.6 kDa. MAPK1 is purified by using an optimized multi-step FPLC method for maximum separation from contaminants. | |
Limitations and Performance Guarantee
enQuire Bio’s products are guaranteed and approved for RESEARCH USE ONLY or manufacturing purposes. This product may not be used as a pharmaceutical agent, or other product intended for human consumption without meeting additional regulatory requirements. This product is guaranteed to work for a period of two years when stored at -70C or colder, and one year when aliquoted and stored at -20C.
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